Center for Ecology, Southern Illinois University Carbondale, 1125 Lincoln Drive, Carbondale, IL 62901, USA.
Department of Microbiology, Southern Illinois University Carbondale, 1125 Lincoln Drive, Carbondale, IL 62901, USA.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):189-194. doi: 10.1099/ijs.0.064477-0. Epub 2014 Oct 16.
Enrichment cultures inoculated with hydrothermally influenced nearshore sediment from Papua New Guinea led to the isolation of an arsenic-tolerant, acidophilic, facultatively aerobic bacterial strain designated PNG-April(T). Cells of this strain were Gram-stain-negative, rod-shaped, motile and did not form spores. Strain PNG-April(T) grew at temperatures between 4 °C and 40 °C (optimum 30-37 °C), at pH 3.5 to 8.3 (optimum pH 5-6) and in the presence of up to 2.7% NaCl (optimum 0-1.0%). Both arsenate and arsenite were tolerated up to concentrations of at least 0.5 mM. Metabolism in strain PNG-April(T) was strictly respiratory. Heterotrophic growth occurred with O2 or nitrate as electron acceptors, and aerobic lithoautotrophic growth was observed with thiosulfate or nitrite as electron donors. The novel isolate was capable of N2-fixation. The respiratory quinones were Q-8 and Q-7. Phylogenetically, strain PNG-April(T) belongs to the genus Burkholderia and shares the highest 16S rRNA gene sequence similarity with the type strains of Burkholderia fungorum (99.8%), Burkholderia phytofirmans (98.8%), Burkholderia caledonica (98.4%) and Burkholderia sediminicola (98.4%). Differences from these related species in several physiological characteristics (lipid composition, carbohydrate utilization, enzyme profiles) and DNA-DNA hybridization suggested the isolate represents a novel species of the genus Burkholderia, for which we propose the name Burkholderia insulsa sp. nov. The type strain is PNG-April(T) ( = DSM 28142(T) = LMG 28183(T)).
从巴布亚新几内亚受热水影响的近岸沉积物中富集培养物，导致了耐砷、嗜酸、兼性需氧细菌菌株 PNG-April(T)的分离。该菌株的细胞革兰氏阴性，杆状，运动，不形成孢子。PNG-April(T) 菌株在 4-40°C（最适 30-37°C）之间的温度下生长，在 pH 值为 3.5-8.3（最适 pH 值为 5-6）的条件下生长，并且在高达 2.7%的 NaCl（最适 0-1.0%）的存在下生长。砷酸盐和亚砷酸盐都能耐受至少 0.5mM 的浓度。PNG-April(T) 菌株的代谢是严格的呼吸作用。在以 O2 或硝酸盐作为电子受体的异养生长中，观察到以硫代硫酸盐或亚硝酸盐作为电子供体的好氧自养生长。新型分离株能够进行 N2 固定。呼吸醌为 Q-8 和 Q-7。在系统发育上，PNG-April(T) 菌株属于伯克霍尔德氏菌属，与伯克霍尔德氏菌真菌（99.8%）、伯克霍尔德氏菌植物亚种（98.8%）、伯克霍尔德氏菌喀里多尼亚亚种（98.4%）和伯克霍尔德氏菌底泥亚种（98.4%）的模式菌株的 16S rRNA 基因序列相似度最高。与这些相关种在几种生理特性（脂类组成、碳水化合物利用、酶谱）和 DNA-DNA 杂交方面的差异表明，该分离株代表了伯克霍尔德氏菌属的一个新种，我们建议将其命名为 Burkholderia insulsa sp. nov. 模式菌株为 PNG-April(T)（=DSM 28142(T)=LMG 28183(T)）。
