Wang Fangbin, Yuan Junhua, Berg Howard C
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, 230026, China; and.
Hefei National Laboratory for Physical Sciences at the Microscale and Department of Physics, University of Science and Technology of China, Hefei, Anhui, 230026, China; and
Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):15752-5. doi: 10.1073/pnas.1418548111. Epub 2014 Oct 20.
Switching dynamics of flagellar motors of Escherichia coli is commonly observed through markers attached to the flagellar filaments. To eliminate possible complications resulting from the conformational transitions of these filaments and to look at the output of motors more directly, we monitored motor rotation by attaching nanogold spheres to the hooks of cells lacking filaments. We observed exponentially distributed counterclockwise (CCW) and clockwise (CW) intervals and Lorentzian power spectra of the switching time series consistent with models that treat motor switching as a two-state Poisson process.
通过附着在鞭毛丝上的标记物,通常可以观察到大肠杆菌鞭毛马达的切换动力学。为了消除这些丝状体构象转变可能带来的并发症,并更直接地观察马达的输出,我们通过将纳米金球附着在缺乏丝状体的细胞的钩上来监测马达旋转。我们观察到逆时针(CCW)和顺时针(CW)间隔呈指数分布,以及切换时间序列的洛伦兹功率谱,这与将马达切换视为两态泊松过程的模型一致。