Snider Chloe E, Stephens Andrew D, Kirkland Jacob G, Hamdani Omar, Kamakaka Rohinton T, Bloom Kerry
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.
Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064.
J Cell Biol. 2014 Oct 27;207(2):189-99. doi: 10.1083/jcb.201405028. Epub 2014 Oct 20.
Condensin is enriched in the pericentromere of budding yeast chromosomes where it is constrained to the spindle axis in metaphase. Pericentric condensin contributes to chromatin compaction, resistance to microtubule-based spindle forces, and spindle length and variance regulation. Condensin is clustered along the spindle axis in a heterogeneous fashion. We demonstrate that pericentric enrichment of condensin is mediated by interactions with transfer ribonucleic acid (tRNA) genes and their regulatory factors. This recruitment is important for generating axial tension on the pericentromere and coordinating movement between pericentromeres from different chromosomes. The interaction between condensin and tRNA genes in the pericentromere reveals a feature of yeast centromeres that has profound implications for the function and evolution of mitotic segregation mechanisms.
凝缩蛋白在出芽酵母染色体的着丝粒周围富集，在中期它被限制在纺锤体轴上。着丝粒周围的凝缩蛋白有助于染色质压缩、抵抗基于微管的纺锤体力量以及纺锤体长度和变异调节。凝缩蛋白沿着纺锤体轴以异质方式聚集。我们证明凝缩蛋白在着丝粒周围的富集是由与转运核糖核酸（tRNA）基因及其调控因子的相互作用介导的。这种招募对于在着丝粒上产生轴向张力以及协调来自不同染色体的着丝粒之间的运动很重要。着丝粒中凝缩蛋白与tRNA基因之间的相互作用揭示了酵母着丝粒的一个特征，这对有丝分裂分离机制的功能和进化具有深远影响。
