伊朗南部设拉子临床分离株中 VanA 和 VanB 阳性耐万古霉素金黄色葡萄球菌
VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran.
作者信息
Saadat Sareh, Solhjoo Kavous, Norooz-Nejad Mohammad-Javad, Kazemi Akbar
机构信息
Instructor Microbiology Department, Islamic Azad University, Jahrom Branch (the member of young scientific research club of Islamic Azad university of Jahrom), Jahrom, Iran.
Assistant Professor Medical Microbiology Department, School of Medicine, Jahrom University of Medical Sciences, Motahri Street, Jahrom, Iran.
出版信息
Oman Med J. 2014 Sep;29(5):335-9. doi: 10.5001/omj.2014.90.
OBJECTIVE
The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals.
METHODS
From March to December 2012, 100 S. aureus isolates (mainly from wound and blood) were collected from three hospitals in Shiraz, south of Iran. After identification of Staphylococcus aureus by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion test for 13 different antibiotics. Vancomycin-resistant Staphylococcus aureus isolates were determined by vancomycin agar screening test and PCR for vancomycin resistant genes (vanA and vanB).
RESULTS
The lowest and highest resistance was seen for quinupristin-dalfopristin (n=1) and ampicillin (n=95), respectively. Vancomycin agar screening test showed that 37 isolates can grow on these media. Further study by PCR also detected vanA and/or vanB genes in all of these strains. Also, 19 isolates showed either vanA or vanB but were susceptible according to vancomycin agar screening test. In total, vanA and vanB resistant genes were detected in 34% and 37% of clinical isolates, respectively.
CONCLUSION
The results showed that the frequency of vancomycin resistance genes (vanA, vanB) is very high in Staphylococcus aureus strains isolated from patients in south of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum.
目的
本研究旨在确定从设拉子医院临床样本中分离出的耐万古霉素金黄色葡萄球菌的患病率。
方法
2012年3月至12月，从伊朗南部设拉子的三家医院收集了100株金黄色葡萄球菌分离株（主要来自伤口和血液）。通过生化、微生物学和分子方法鉴定金黄色葡萄球菌后，采用 Kirby-Bauer 纸片扩散法对13种不同抗生素进行药敏试验。通过万古霉素琼脂筛选试验和针对万古霉素耐药基因（vanA和vanB）的PCR检测耐万古霉素金黄色葡萄球菌分离株。
结果
对奎奴普丁-达福普汀（n = 1）和氨苄西林（n = 95）的耐药率分别为最低和最高。万古霉素琼脂筛选试验表明，37株分离株可在这些培养基上生长。PCR进一步研究还在所有这些菌株中检测到vanA和/或vanB基因。此外，19株分离株显示有vanA或vanB，但根据万古霉素琼脂筛选试验为敏感。总共在34%和37%的临床分离株中分别检测到vanA和vanB耐药基因。
结论
结果表明，从伊朗南部患者中分离出的金黄色葡萄球菌菌株中，万古霉素耐药基因（vanA、vanB）的频率非常高。因此，需要采取紧急干预措施，将这些分离株的出现和传播降至最低。
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