Chattopadhyay Sourav, Dey Sankar Kumar, Maiti Prasanta Kumar, Dolai Durgapada
Department of Physiology, Immunology and Microbiology Laboratory, S. B. S. S. Mahavidyalaya (Affiliated to Vidyasagar University), Paschim Midnapore, West Bengal, India.
J Biol Inorg Chem. 2014 Dec;19(8):1377-84. doi: 10.1007/s00775-014-1199-1. Epub 2014 Oct 22.
This work investigated a simple and versatile modification to a solid substrate to develop antibody recognition using nanoparticles. The new immobilized metal ion affinity adsorbent containing nanoparticles and hydrophilic resins is proposed here to improve the binding of antigen on its surface. The light-scattering properties of submicroscopic metal particles ranging from 100 to 120 nm in diameter were confirmed by scanning electron microscopy. We found that synthesized nanoparticles have an inherent enzyme mimetic activity similar to that found in natural peroxidases. The synthesized nanoparticles were coated with Salmonella typhi and Salmonella paratyphi antigens and were allowed to react with Salmonella-infected serum. Positive reactions were detected visually with the naked eye. The color changes of substrate (TMB) were observed even in 1:800 dilutions. This report helps in developing a specific immunoassay using nano-conjugated antigen for the rapid detection of S. typhi and S. paratyphi antibodies in infected serum.
本研究通过对固体基质进行简单且通用的修饰，利用纳米颗粒实现抗体识别。本文提出了一种含有纳米颗粒和亲水树脂的新型固定化金属离子亲和吸附剂，以改善抗原在其表面的结合。通过扫描电子显微镜证实了直径为100至120纳米的亚微观金属颗粒的光散射特性。我们发现合成的纳米颗粒具有与天然过氧化物酶相似的固有酶模拟活性。合成的纳米颗粒用伤寒沙门氏菌和副伤寒沙门氏菌抗原包被，并使其与感染沙门氏菌的血清反应。肉眼可直观检测到阳性反应。即使在1:800稀释度下也能观察到底物（TMB）的颜色变化。本报告有助于开发一种使用纳米缀合抗原的特异性免疫测定方法，用于快速检测感染血清中的伤寒沙门氏菌和副伤寒沙门氏菌抗体。
