1] Centre de Biologie du Développement, Université de Toulouse, UPS, 31062 Toulouse cedex 9, France [2] CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, 31062 cedex 9, France.
Okazaki Institute for Integrative Bioscience, National institutes of Natural Sciences Okazaki, Aichi 444-8787, Japan.
Nat Cell Biol. 2014 Nov;16(11):1035-44. doi: 10.1038/ncb3052. Epub 2014 Oct 26.
Animal development fundamentally relies on the precise control, in space and time, of genome expression. Whereas we have a wealth of information about spatial patterning, the mechanisms underlying temporal control remain poorly understood. Here we show that Pri peptides, encoded by small open reading frames, are direct mediators of the steroid hormone ecdysone for the timing of developmental programs in Drosophila. We identify a previously uncharacterized enzyme of ecdysone biosynthesis, GstE14, and find that ecdysone triggers pri expression to define the onset of epidermal trichome development, through post-translational control of the Shavenbaby transcription factor. We show that manipulating pri expression is sufficient to either put on hold or induce premature differentiation of trichomes. Furthermore, we find that ecdysone-dependent regulation of pri is not restricted to epidermis and occurs over various tissues and times. Together, these findings provide a molecular framework to explain how systemic hormonal control coordinates specific programs of differentiation with developmental timing.
动物的发育从根本上依赖于基因组表达在空间和时间上的精确控制。尽管我们已经有了大量关于空间模式的信息，但时间控制的机制仍知之甚少。在这里，我们发现 Pri 肽是由小开放阅读框编码的，是类固醇激素蜕皮激素调节果蝇发育程序时间的直接介质。我们鉴定了一个以前未被表征的蜕皮激素生物合成酶 GstE14，并发现蜕皮激素通过 Shavenbaby 转录因子的翻译后控制触发 pri 表达，从而定义表皮刚毛发育的开始。我们表明，操纵 pri 表达足以暂停或诱导刚毛的过早分化。此外，我们发现，蜕皮激素依赖的 pri 调节不仅限于表皮，而且发生在各种组织和时间。总之，这些发现为解释系统激素控制如何协调特定的分化程序与发育时间提供了一个分子框架。
