Seafood Technology Division, Fisheries Research Institute, Council of Agriculture, Keelung City, Taiwan, ROC.
Cytotechnology. 2015 Aug;67(4):653-60. doi: 10.1007/s10616-014-9745-9. Epub 2014 Oct 26.
Diabetes is one of the most prevalent chronic diseases globally. In this study, major polyphenols (17.35 ± 0.93-36.66 ± 2.01 mg/g) and minor fucoxanthin (non detected 15.12 ± 0.09 mg/g) were isolated from water, ethanol, and acetone extracts (WES, EES, and AES, respectively) of Sargassum hemiphyllum. Inhibition of α-amylase, α-glucosidase, sucrose, and maltase activities and stimulation of insulin secretion was greater with AES than with WES or EES and correlated with polyphenol and fucoxanthin concentrations in extracts. Moreover, 250 μg/ml EES and AES significantly increased insulin secretion in the presence of 25 mg/ml glibenclamide to higher levels than those obtained with 50 mg/ml glibenclamide. None of the extracts exhibited cytotoxicity, exacerbated the side effects of glibenclamide, or inhibited glibenclamide-induced insulin secretion. These results suggested that the S. hemiphyllum extracts WES, EES, and AES could be used as pharmaceuticals and functional foods to reduce dosages of synthetic diabetes drugs.
糖尿病是全球最普遍的慢性疾病之一。在这项研究中，从半叶马尾藻的水提物、醇提物和丙酮提物（分别为 WES、EES 和 AES）中分离出了主要多酚（17.35 ± 0.93-36.66 ± 2.01 mg/g）和少量岩藻黄质（未检出 15.12 ± 0.09 mg/g）。AES 对α-淀粉酶、α-葡萄糖苷酶、蔗糖和麦芽糖活性的抑制作用以及对胰岛素分泌的刺激作用均大于 WES 或 EES，且与提取物中多酚和岩藻黄质的浓度相关。此外，250μg/ml 的 EES 和 AES 能在 25mg/ml 格列本脲存在的情况下显著增加胰岛素分泌，使其水平高于 50mg/ml 格列本脲的水平。这些提取物均无细胞毒性，也不会加重格列本脲的副作用，或抑制格列本脲诱导的胰岛素分泌。这些结果表明，半叶马尾藻的 WES、EES 和 AES 提取物可用作药物和功能性食品，以减少合成糖尿病药物的剂量。
