Yu Guoqin, Dye Bruce A, Gail Mitchell H, Shi Jianxin, Klepac-Ceraj Vanja, Paster Bruce J, Wang Guo-Qing, Wei Wen-Qiang, Fan Jin-Hu, Qiao You-Lin, Dawsey Sanford M, Freedman Neal D, Abnet Christian C
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Rockville, MD, USA.
BMC Public Health. 2014 Oct 27;14:1110. doi: 10.1186/1471-2458-14-1110.
Bacteria affect oral health, but few studies have systematically examined the role of bacterial communities in oral diseases. We examined this relationship in a large population-based Chinese cancer screening cohort.
Human Oral Microbe Identification Microarrays were used to test for the presence of 272 human oral bacterial species (97 genera) in upper digestive tract (UDT) samples collected from 659 participants. Oral health was assessed using US NHANES (National Health and Nutrition Examination Survey) protocols. We assessed both dental health (total teeth missing; tooth decay; and the decayed, missing, and filled teeth (DMFT) score) and periodontal health (bleeding on probing (BoP) extent score, loss of attachment extent score, and a periodontitis summary estimate).
Microbial richness, estimated by number of genera per sample, was positively correlated with BoP score (P = 0.015), but negatively correlated with tooth decay and DMFT score (P = 0.008 and 0.022 respectively). Regarding β-diversity, as estimated by the UniFrac distance matrix for pairwise differences among samples, at least one of the first three principal components of the UniFrac distance matrix was correlated with the number of missing teeth, tooth decay, DMFT, BoP, or periodontitis. Of the examined genera, Parvimonas was positively associated with BoP and periodontitis. Veillonellacease [G-1] was associated with a high DMFT score, and Filifactor and Peptostreptococcus were associated with a low DMFT score.
Our results suggest distinct relationships between UDT microbiota and dental and periodontal health. Poor dental health was associated with a less microbial diversity, whereas poor periodontal health was associated with more diversity and the presence of potentially pathogenic species.
细菌会影响口腔健康，但很少有研究系统地探究细菌群落在口腔疾病中的作用。我们在一个基于人群的大型中国癌症筛查队列中研究了这种关系。
使用人类口腔微生物鉴定微阵列检测从659名参与者收集的上消化道（UDT）样本中272种人类口腔细菌物种（97个属）的存在情况。采用美国国家健康与营养检查调查（NHANES）方案评估口腔健康状况。我们评估了牙齿健康（缺失牙总数、龋齿以及龋失补牙（DMFT）评分）和牙周健康状况（探诊出血（BoP）程度评分、附着丧失程度评分以及牙周炎综合评估）。
通过每个样本中的属数量估算的微生物丰富度与BoP评分呈正相关（P = 0.015），但与龋齿和DMFT评分呈负相关（分别为P = 0.008和0.022）。关于β多样性，通过样本间成对差异的UniFrac距离矩阵估算，UniFrac距离矩阵的前三个主成分中至少有一个与缺失牙数量、龋齿、DMFT、BoP或牙周炎相关。在所检测的属中，小单胞菌属与BoP和牙周炎呈正相关。韦荣球菌科[G-1]与高DMFT评分相关，而纤细杆菌属和消化链球菌属与低DMFT评分相关。
我们的结果表明UDT微生物群与牙齿和牙周健康之间存在不同的关系。牙齿健康状况不佳与微生物多样性较低有关，而牙周健康状况不佳与更多样性以及潜在致病物种的存在有关。
