Andereggen Lukas, Neuschmelting Volker, von Gunten Michael, Widmer Hans Rudolf, Takala Jukka, Jakob Stephan M, Fandino Javier, Marbacher Serge
Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital; Harvard Medical School, Boston Children's Hospital; Department of Neurosurgery, University and Bern University Hospital (Inselspital).
Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital); Department of Neurosurgery, University Hospital Cologne.
J Vis Exp. 2014 Oct 2(92):e52132. doi: 10.3791/52132.
Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and controllable animal models that simulate both conditions are presently uncommon. Therefore, new models are needed in order to mimic human pathophysiological conditions resulting from SAH. This report describes the technical nuances of a rabbit blood-shunt SAH model that enables control of intracerebral pressure (ICP). An extracorporeal shunt is placed between the arterial system and the subarachnoid space, which enables examiner-independent SAH in a closed cranium. Step-by-step procedural instructions and necessary equipment are described, as well as technical considerations to produce the model with minimal mortality and morbidity. Important details required for successful surgical creation of this robust, simple and consistent ICP-controlled SAH rabbit model are described.
早期脑损伤和迟发性脑血管痉挛均会导致蛛网膜下腔出血（SAH）后出现不良预后。目前，能够同时模拟这两种情况的可重复且可控的动物模型并不常见。因此，需要新的模型来模拟SAH导致的人类病理生理状况。本报告描述了一种能够控制颅内压（ICP）的兔血液分流SAH模型的技术细节。在动脉系统和蛛网膜下腔之间放置一个体外分流装置，这使得在封闭的颅骨内能够进行与检查者无关的SAH操作。文中描述了分步操作说明和所需设备，以及以最低死亡率和发病率建立该模型的技术考量。还介绍了成功手术创建这种强大、简单且一致的ICP控制SAH兔模型所需的重要细节。
