Juntavee Apa, Peerapattana Jomjai, Ratanathongkam Ariya, Nualkaew Nartsajee, Chatchiwiwattana Supaporn, Treesuwan Panta
Associate Professor, Department of Pediatric Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
Associate Professor, Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen Thailand.
Int J Clin Pediatr Dent. 2014 May;7(2):77-81. doi: 10.5005/jp-journals-10005-1241. Epub 2014 Aug 29.
New approaches for chemomechanical caries removal require effective materials with antibacterial properties for removal of infected dentin. Apacaries gel is a newly developed material comprised polyphenol from mangosteen extracts and papain mixed in gel preparation.
This study evaluated the antibacterial effects of Apacaries gel on Streptococcus mutans in vitro.
Mangosteen pericarp powder was extracted. The amount of phenolic compounds was determined using the Folin-Ciocalteu method. The time-kill kinetics were investigated. Mangosteen extract and papain were mixed with gel base to develop Apacaries gel. The inhibition zone of the Apacaries gel was determined using agar well diffusion methods.
The mangosteen pericarp extract, which contains α-mangostin, was active against S. mutans strain ATCC25175. The time-kill kinetics curve showed that applying 1 mg/ml of mangosteen extract can reduce S. mutans by 50% within approximately 5 seconds; after this reduction, the bacterial count rapidly dropped to 0 within 60 seconds. Using mangosteen extract and papain mixture gel preparation resulted in a larger inhibition zone than using the mangosteen extract gel or papain gel separately.
Apacaries gel can effectively inhibit S. mutans strain ATCC25175. Apacaries is capable of S. mutans inhibition better than both mangosteen extract or papain separately. How to cite this article: Juntavee A, Peerapattana J, Ratanathongkam A, Nualkaew N, Chatchiwiwattana S, Treesuwan P. The Antibacterial Effects of Apacaries Gel on Streptococcus mutans: An in vitro Study. Int J Clin Pediatr Dent 2014;7(2):77-81.
化学机械性龋损去除的新方法需要具有抗菌性能的有效材料来去除感染的牙本质。Apacaries凝胶是一种新开发的材料，由山竹提取物中的多酚和木瓜蛋白酶混合制成凝胶制剂。
本研究评估Apacaries凝胶在体外对变形链球菌的抗菌作用。
提取山竹果皮粉末。采用福林-酚法测定酚类化合物的含量。研究时间-杀菌动力学。将山竹提取物和木瓜蛋白酶与凝胶基质混合以制备Apacaries凝胶。采用琼脂孔扩散法测定Apacaries凝胶的抑菌圈。
含有α-倒捻子素的山竹果皮提取物对变形链球菌ATCC25175菌株有活性。时间-杀菌动力学曲线显示，应用1mg/ml的山竹提取物可在约5秒内使变形链球菌减少50%；在此减少之后，细菌数量在60秒内迅速降至0。使用山竹提取物和木瓜蛋白酶混合凝胶制剂产生的抑菌圈比单独使用山竹提取物凝胶或木瓜蛋白酶凝胶更大。
Apacaries凝胶可有效抑制变形链球菌ATCC25175菌株。Apacaries对变形链球菌的抑制能力优于单独的山竹提取物或木瓜蛋白酶。如何引用本文：Juntavee A, Peerapattana J, Ratanathongkam A, Nualkaew N, Chatchiwiwattana S, Treesuwan P. Apacaries凝胶对变形链球菌的抗菌作用：一项体外研究。《国际临床儿科牙科学杂志》2014；7(2):77-81。
