Yu Zheng-Zheng, Chen Jing, Shou Pei-Qin, Feng Lei
Ningbo College of Health Sciences Yinzhouquxuefu Road No. 51, Ningbo 315100, Zhejiang, China.
Zhejiang Agricultural University Lin'anshihuanchengbei Road, Hangzhou 311300, Zhejiang, China.
Int J Clin Exp Med. 2014 Sep 15;7(9):2754-62. eCollection 2014.
Male infertility is a serious global medical and social issue demanding more specific and effective treatments. In this study, we generated a male infertility rat model using adenine induction to study the effects of certain micronutrients on reproduction. Fifty male SD rats were used in the study, and thirty of them received daily intra-gastric administration of 300 mg/kg adenine for four weeks. The thirty adenine treated mice were evenly divided into 3 groups to receive intra-gastric administration of micronutrient mixture of vitamin A, vitamin C, vitamin E, Zinc, and selenium (micronutrient group), normal saline (model control group), and methyl testosterone solution (androgen group). The other twenty rats used were normal male SD rats that were evenly divided into two groups to receive intra-gastric administration of normal saline (normal control group) and micronutrient mixture first then adenine 40 min later (micronutrient prevention group). After four weeks of micronutrient and other treatments, all rats were sacrificed for analyses. Compared with those in the model control group, the rats in the micronutrient group showed significantly improved physical signs, significantly increased body weights, significantly increased testis index, significantly increased sperm counts and motility, significantly decreased sperm malformation, and significantly repaired testis tissue. Compared with those in the model control group, the rats in the micronutrient group showed significantly decreased FSH levels and recovered LH and testosterone levels. The rats in the micronutrient prevention group did not show significant differences in sperm counts, sperm motility, sperm malformation, and hormonal levels from those in the normal control group. The findings from this study provide evidence for the potential application of micronutrients in male infertility treatments.
男性不育是一个严重的全球性医学和社会问题，需要更具针对性和有效性的治疗方法。在本研究中，我们使用腺嘌呤诱导建立了男性不育大鼠模型，以研究某些微量营养素对生殖的影响。本研究使用了50只雄性SD大鼠，其中30只每天接受300mg/kg腺嘌呤灌胃，持续四周。这30只接受腺嘌呤处理的小鼠被平均分为3组，分别接受维生素A、维生素C、维生素E、锌和硒的微量营养素混合物灌胃（微量营养素组）、生理盐水灌胃（模型对照组）和甲基睾酮溶液灌胃（雄激素组）。另外20只大鼠为正常雄性SD大鼠，它们被平均分为两组，分别接受生理盐水灌胃（正常对照组）和先给予微量营养素混合物灌胃，40分钟后再给予腺嘌呤灌胃（微量营养素预防组）。经过四周的微量营养素及其他处理后，所有大鼠均被处死进行分析。与模型对照组相比，微量营养素组大鼠的身体体征明显改善，体重显著增加，睾丸指数显著升高，精子数量和活力显著增加，精子畸形率显著降低，睾丸组织得到显著修复。与模型对照组相比，微量营养素组大鼠的促卵泡生成素（FSH）水平显著降低，促黄体生成素（LH）和睾酮水平恢复正常。微量营养素预防组大鼠的精子数量、精子活力、精子畸形率和激素水平与正常对照组相比无显著差异。本研究结果为微量营养素在男性不育治疗中的潜在应用提供了证据。
