Stephanopoulos D E, Myers M G, Bernstein D I
Division of Infectious Diseases, Children's Hospital Research Foundation, Cincinnati, Ohio.
J Infect Dis. 1989 Jan;159(1):89-95. doi: 10.1093/infdis/159.1.89.
A small-animal model of genital herpes simplex virus (HSV) infection has not been available for investigating the pathogenesis of disease in males. Male Hartley guinea pigs were inoculated on the medial thigh with 10(5)-10(6) plaque-forming units of HSV type 2. Animals developed vesicular lesions in the genital area, including the penis. Transient urinary retention, hindlimb paralysis, and acute urethral shedding of HSV also occurred. During acute infection, virus was detected in neural and genital tissue homogenates, including the peripheral nerve, lumbosacral dorsal root ganglia (DRG), spinal cord, brain, vas deferens, epididymis, testes, seminal vesicle, and prostate. Recurrent disease developed in all animals. Persistent virus was detected in DRG but rarely in the vas deferens, seminal vesicle, epididymis, and testes. This model produces disease similar to that seen in men and should prove useful for studying the pathogenesis and immunology of male genital HSV type 2 infections.
目前尚无用于研究男性疾病发病机制的单纯疱疹病毒（HSV）感染的小动物模型。将10(5)-10(6)个2型单纯疱疹病毒空斑形成单位接种于雄性哈特利豚鼠的大腿内侧。动物在生殖器区域，包括阴茎，出现水疱性病变。还发生了短暂性尿潴留、后肢麻痹以及HSV的急性尿道排出。在急性感染期间，在神经和生殖器组织匀浆中检测到病毒，包括外周神经、腰骶背根神经节（DRG）、脊髓、脑、输精管、附睾、睾丸、精囊和前列腺。所有动物均出现复发性疾病。在DRG中检测到持续性病毒，但在输精管、精囊、附睾和睾丸中很少检测到。该模型产生的疾病与男性所见相似，应证明对研究男性生殖器2型HSV感染的发病机制和免疫学有用。
