Li Jing, Wang Yuan, Yu Jinjin, Dong Ruofan, Qiu Haifeng
Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Tumour Biol. 2015 Mar;36(3):1661-5. doi: 10.1007/s13277-014-2765-4. Epub 2014 Oct 31.
We recently found that HOTAIR (HOX antisense intergenic RNA) promotes development and induces radioresistance in cervical cancer. In the present study, we investigated the circulating HOTAIR expression and determined its relationships with the clinicopathological parameters in cervical cancer. The sera samples were obtained from 118 pathological diagnosed cervical cancer patients and 100 normal age-matched women. The expression of HOTAIR was measured by quantitative real time PCR. Patients' information were collected and analyzed by the SPSS 17.0 software. Compared with normal control, the expression of HOTAIR was significantly upregulated in the sera of cervical cancer patients (P < 0.0001). In addition, elevated HOTAIR was associated with advanced tumor stages (P < 0.0001), adenocarcinoma (P < 0.0001), lymphatic vascular space invasion (P = 0.0065), and lymphatic node metastasis (P = 0.0259). In addition, our follow-up data showed that high HOTAIR was notably correlated with tumor recurrence (P = 0.013) and short overall survival (P = 0.009). Circulating HOTAIR was commonly upregulated and a potent prognostic marker in cervical cancer.
我们最近发现，HOTAIR（HOX反义基因间RNA）可促进宫颈癌的发展并诱导其放射抗性。在本研究中，我们调查了循环中HOTAIR的表达情况，并确定了其与宫颈癌临床病理参数的关系。血清样本取自118例经病理诊断的宫颈癌患者和100例年龄匹配的正常女性。采用定量实时PCR检测HOTAIR的表达。通过SPSS 17.0软件收集并分析患者信息。与正常对照相比，宫颈癌患者血清中HOTAIR的表达显著上调（P < 0.0001）。此外，HOTAIR升高与肿瘤晚期（P < 0.0001）、腺癌（P < 0.0001）、淋巴管间隙浸润（P = 0.0065）及淋巴结转移（P = 0.0259）相关。另外，我们的随访数据显示，高HOTAIR水平与肿瘤复发（P = 0.013）及总生存期短（P = 0.009）显著相关。循环中的HOTAIR在宫颈癌中普遍上调，是一种有效的预后标志物。
