五种酶免疫测定法、电子显微镜检查法和乳胶凝集法检测粪便标本中轮状病毒的比较
Comparison of five enzyme immunoassays, electron microscopy, and latex agglutination for detection of rotavirus in fecal specimens.
作者信息
Kok T W, Burrell C J
机构信息
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, Australia.
出版信息
J Clin Microbiol. 1989 Feb;27(2):364-6. doi: 10.1128/jcm.27.2.364-366.1989.
Abstract
Five different enzyme immunoassays, electron microscopy, and latex agglutination (Slidex; bioMerieux) were compared for the rapid detection of human rotavirus in fecal specimens. The enzyme immunoassay using rotavirus polyclonal antiserum (Dakopatts) with simple in-house modifications was shown by the use of confirmatory tests to be the most sensitive and specific procedure.
摘要
对五种不同的酶免疫测定法、电子显微镜检查法和乳胶凝集试验（Slidex；生物梅里埃公司）进行了比较，以快速检测粪便标本中的人轮状病毒。通过使用确证试验表明，对使用轮状病毒多克隆抗血清（达科帕茨公司）并经简单内部改良的酶免疫测定法是最敏感和特异的方法。
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