An Fang, Yang Guodong, Tian Jiaming, Wang Shuhua
College of Pharmacy, Hebei North University, Zhangjiakou 075000, Hebei Province, China.
Neural Regen Res. 2012 Nov 25;7(33):2565-75. doi: 10.3969/j.issn.1673-5374.2012.33.001.
Total flavonoids are the main pharmaceutical components of Trollius chinensis Bunge, and orientin and vitexin are the monomer components of total flavonoids in Trollius chinensis Bunge. In this study, an aged mouse model was established through intraperitoneal injection of D-galactose for 8 weeks, followed by treatment with 40, 20, or 10 mg/kg orientin, vitexin, or a positive control (vitamin E) via intragastric administration for an additional 8 weeks. Orientin, vitexin, and vitamin E improved the general medical status of the aging mice and significantly increased their brain weights. They also produced an obvious rise in total antioxidant capacity, superoxide dismutase, catalase, and glutathione peroxidase levels in the serum, and the levels of superoxide dismutase, catalase and glutathione peroxidase, Na(+)-K(+)-ATP enzyme, and Ca(2+)-Mg(2+)-ATP enzyme in the liver, brain and kidneys. In addition, they significantly reduced malondialdehyde levels in the liver, brain and kidney and lipofuscin levels in the brain. They also significantly improved the neuronal ultrastructure. The 40 mg/kg dose of orientin and vitexin had the same antioxidant capacity as vitamin E. These experimental findings indicate that orientin and vitexin engender anti-aging effects through their antioxidant capacities.
总黄酮是金莲花的主要药用成分，而荭草苷和牡荆素是金莲花总黄酮中的单体成分。在本研究中，通过腹腔注射D-半乳糖8周建立衰老小鼠模型，随后通过灌胃给予40、20或10mg/kg的荭草苷、牡荆素或阳性对照（维生素E），持续8周。荭草苷、牡荆素和维生素E改善了衰老小鼠的总体健康状况，并显著增加了它们的脑重量。它们还使血清中的总抗氧化能力、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平明显升高，以及肝脏、大脑和肾脏中的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、Na(+)-K(+)-ATP酶和Ca(2+)-Mg(2+)-ATP酶水平升高。此外，它们显著降低了肝脏、大脑和肾脏中的丙二醛水平以及大脑中的脂褐素水平。它们还显著改善了神经元超微结构。40mg/kg剂量的荭草苷和牡荆素具有与维生素E相同的抗氧化能力。这些实验结果表明，荭草苷和牡荆素通过其抗氧化能力产生抗衰老作用。
