Li Sixing, Ding Xiaoyun, Mao Zhangming, Chen Yuchao, Nama Nitesh, Guo Feng, Li Peng, Wang Lin, Cameron Craig E, Huang Tony Jun
Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
Lab Chip. 2015 Jan 7;15(1):331-8. doi: 10.1039/c4lc00903g.
Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.
细胞/微珠清洗是各种细胞研究和分析过程中不可或缺的样品制备程序。在本文中，我们报道了一种基于驻波表面声波（SSAW）的微流控装置，用于连续流动中的细胞和微珠清洗。在我们的方法中，利用SSAW场中产生的声辐射力从其原始培养基中主动提取细胞或微珠。我们的装置采用了独特的倾斜角驻波表面声波（taSSAW）配置，使我们能够以>98%的回收率和>97%的清洗效率清洗微珠。我们还通过细胞清洗从裂解的血液样本中制备高纯度（>97%）白细胞，展示了我们装置的功能。我们基于SSAW的细胞/微珠清洗装置具有无标记操作、简单、高生物相容性、高回收率和高清洗效率等优点。它可用于许多芯片实验室应用。
