Flomenbaum M, Soeiro R, Udem S A, Kress Y, Factor S M
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York.
J Acquir Immune Defic Syndr (1988). 1989;2(2):129-35.
In order to determine if cardiac tissue from AIDS patients or patients with seropositivity to HIV-1 might be infected by HIV-1, portions of myocardium obtained postmortem were evaluated for HIV-1 DNA sequences. Cellular DNA was extracted and digested with EcoR1 and Southern blots were performed. One of three AIDS hearts was positive for HIV-1 DNA sequences without amplification, whereas two additional hearts were positive for HIV-1 DNA after amplification. Accordingly, other tissue from the heart positive for HIV-1 without amplification was studied by electron microscopy to localize HIV virions. Unexpectedly, large numbers of proliferating multilamellated membrane bodies were identified in myocytes, predominantly associated with mitochondria. Identical membrane bodies were found in two additional AIDS hearts, and in one heart from a patient with seropositivity to the AIDS virus, but in none of three similarly fixed controls. Immunocytochemistry for HIV core (p24) and envelope (gp120) antigens did not localize gold-labeled antibodies to the membrane bodies. We believe this membranopathy may be an HIV-1- or AIDS-specific abnormality of unknown etiology that may be related to the ultimate development of cardiomyopathy. In addition, our studies provide further support that HIV-1 may be present in AIDS hearts, although as yet we cannot state with certainty where the HIV-1 is located in these tissues.
为了确定艾滋病患者或HIV-1血清反应阳性患者的心脏组织是否可能被HIV-1感染，对死后获取的部分心肌进行了HIV-1 DNA序列评估。提取细胞DNA并用EcoR1进行消化，然后进行Southern印迹分析。三个艾滋病患者的心脏中有一个在未扩增的情况下HIV-1 DNA序列呈阳性，而另外两个心脏在扩增后HIV-1 DNA呈阳性。因此，对未扩增的HIV-1阳性心脏的其他组织进行了电子显微镜研究，以定位HIV病毒颗粒。出乎意料的是，在心肌细胞中发现了大量增殖的多层膜体，主要与线粒体相关。在另外两个艾滋病患者的心脏以及一名艾滋病病毒血清反应阳性患者的一个心脏中也发现了相同的膜体，但在三个同样固定的对照心脏中均未发现。针对HIV核心（p24）和包膜（gp120）抗原的免疫细胞化学分析未将金标记抗体定位到膜体上。我们认为这种膜病可能是一种病因不明的HIV-1或艾滋病特异性异常，可能与心肌病的最终发展有关。此外，我们的研究进一步支持了HIV-1可能存在于艾滋病患者的心脏中，尽管目前我们尚不能确定这些组织中HIV-1的位置。
