Crook T, Morgenstern J P, Crawford L, Banks L
Molecular Virology Laboratory, Imperial Cancer Research Fund, London, UK.
EMBO J. 1989 Feb;8(2):513-9. doi: 10.1002/j.1460-2075.1989.tb03405.x.
The close association between HPV-16 and cervical cancer implies some role for the virus in development of this cancer. Recent studies have shown that the HPV-16 E7 gene encodes the major transforming activity of the virus in baby rat kidney (BRK) cell transformation assays. To investigate the requirement for continued E7 expression in BRK cells transformed by HPV-16 E7 plus EJ-ras, we have developed a system for inducible expression of the E7 gene. The studies reported here show that continued expression of the HPV-16 E7 gene is required for maintenance of the transformed phenotype in these cells. The implications these observations bear on the role of the E7 gene in cervical carcinoma are discussed.
人乳头瘤病毒16型（HPV-16）与宫颈癌之间的密切关联暗示了该病毒在这种癌症发展过程中发挥了某种作用。最近的研究表明，在幼鼠肾（BRK）细胞转化试验中，HPV-16 E7基因编码了该病毒的主要转化活性。为了研究在由HPV-16 E7加EJ- ras转化的BRK细胞中持续表达E7的必要性，我们开发了一种用于E7基因诱导表达的系统。此处报道的研究表明，这些细胞中维持转化表型需要持续表达HPV-16 E7基因。本文讨论了这些观察结果对E7基因在宫颈癌中作用的影响。
