Androphy E J, Hubbert N L, Schiller J T, Lowy D R
EMBO J. 1987 Apr;6(4):989-92. doi: 10.1002/j.1460-2075.1987.tb04849.x.
Human cervical carcinoma cell lines that harbor human papillomavirus (HPV) have been reported to retain selectively and express HPV sequences which could encode viral E6 and E7 proteins. The potential importance of HPV E6 to tumors is suggested further by the observation that bovine papillomavirus (BPV) E6 can induce morphologic transformation of mouse cells in vitro. To identify HPV E6 protein, a polypeptide encoded by HPV-16 E6 was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 18-kd protein in two human carcinoma cell lines known to express HPV-16 RNA and in mouse cells morphologically transformed by HPV-16 DNA. The 18-kd E6 protein was distinct from a previously identified HPV-16 E7 protein. The HPV-16 E6 antibodies were found to be type specific in that they did not recognize E6 protein in cells containing HPV-18 sequences and reacted weakly, if at all, to BPV E6 protein. The results demonstrate that human tumors containing HPV-16 DNA can express an E6 protein product. They are consistent with the hypothesis that E6 may contribute to the transformed phenotype in human cervical cancers that express this protein.
据报道，携带人乳头瘤病毒（HPV）的人宫颈癌细胞系能选择性保留并表达HPV序列，这些序列可编码病毒E6和E7蛋白。牛乳头瘤病毒（BPV）E6能在体外诱导小鼠细胞发生形态转化，这一观察结果进一步表明HPV E6对肿瘤具有潜在重要性。为鉴定HPV E6蛋白，一种由HPV - 16 E6编码的多肽在细菌表达载体中产生，并用于制备抗血清。该抗血清在两种已知表达HPV - 16 RNA的人癌细胞系以及被HPV - 16 DNA形态转化的小鼠细胞中，特异性免疫沉淀出预测的18 kDa蛋白。18 kDa的E6蛋白与先前鉴定的HPV - 16 E7蛋白不同。发现HPV - 16 E6抗体具有型特异性，因为它们在含有HPV - 18序列的细胞中不识别E6蛋白，并且对BPV E6蛋白即使有反应也很微弱。结果表明，含有HPV - 16 DNA的人类肿瘤能够表达E6蛋白产物。这些结果与E6可能在表达该蛋白的人宫颈癌中促成转化表型的假说一致。
