大鼠肝脏线粒体的高分辨率31P核磁共振研究。
High-resolution 31P nuclear magnetic resonance study of rat liver mitochondria.
作者信息
Ogawa S, Rottenberg H, Brown T R, Shulman R G, Castillo C L, Glynn P
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1796-800. doi: 10.1073/pnas.75.4.1796.
Intact mitochondria were studied by high-resolution 31P nuclear magnetic resonance. Observable internal phosphate compounds included inorganic phosphate (Pi), ADP, and ATP. The internal pH was determined by the chemical shift of the internal Pi, the pK2 (6.7) of which was measured in uncoupled mitochondria. The observed equilibrium relation between the internal and the external Pi was consistent with the exchange equilibrium through the H2PO4-/OH- carrier. The internal ATP and ADP were essentially Mg2+ bound and their resonances were distinguishable from those of the external ATP and ADP by the chemical shift differences due to the Mg2+ concentration gradient and deltapH. Oxidative phosphorylation was followed by the separate resonances of Pi and adenine nucleotides both internal and external to mitochondria. From these resonances the internal and external phosphate potentials could be estimated.
通过高分辨率31P核磁共振研究完整的线粒体。可观察到的内部磷酸盐化合物包括无机磷酸盐（Pi）、ADP和ATP。内部pH值由内部Pi的化学位移确定，其pK2（6.7）在解偶联线粒体中测量。观察到的内部和外部Pi之间的平衡关系与通过H2PO4-/OH-载体的交换平衡一致。内部ATP和ADP基本上与Mg2+结合，并且由于Mg2+浓度梯度和ΔpH导致的化学位移差异，它们的共振与外部ATP和ADP的共振可区分。线粒体内部和外部的Pi和腺嘌呤核苷酸的单独共振跟踪氧化磷酸化。根据这些共振可以估计内部和外部磷酸盐电位。
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