Department of Emergency Medicine, Changhua Christian Hospital, Changhua, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
School of Public Health, Chung Shan Medical University, Taichung, Taiwan.
J Microbiol Immunol Infect. 2016 Oct;49(5):625-635. doi: 10.1016/j.jmii.2014.08.001. Epub 2014 Nov 11.
Asthma is a common allergic disease. In previous studies, probiotics improved the balance of intestinal microbes, reduced inflammation, and promoted mucosal tolerance. This study investigated whether oral administrations of Lactobacillus rhamnosus GG (LGG) inhibited allergen (ovalbumin or OVA)-induced airway inflammation in a mouse asthma model.
The allergy/asthma animal model in this study was sensitization with OVA. After intranasal challenge with OVA, the airway inflammation and hyper-responsiveness were determined by a Buxco system, bronchoalveolar lavage fluid analysis with Liu stain, and enzyme-linked immunosorbent assay. Histopathologic changes in the lung were detected by hematoxylin and eosin staining and immunohistochemistry staining.
Both pre- and post-treatment with LGG suppressed the airway hyper-responsiveness to methacholine and significantly decreased the number of infiltrating inflammatory cells and Th2 cytokines in bronchoalveolar lavage fluid and serum compared with the OVA-sensitized mice. In addition, LGG reduced OVA-specific IgE levels in serum. Oral LGG decreased matrix metalloproteinase 9 expression in lung tissue and inhibited inflammatory cell infiltration.
LGG had an anti-inflammatory effect on OVA-induced airway inflammation and might be an additional or supplementary therapy for allergic airway diseases.
哮喘是一种常见的过敏性疾病。在以前的研究中，益生菌改善了肠道微生物的平衡，减轻了炎症，并促进了黏膜耐受。本研究探讨了鼠哮喘模型中口服鼠李糖乳杆菌 GG（LGG）是否抑制过敏原（卵清蛋白或 OVA）诱导的气道炎症。
本研究中的过敏/哮喘动物模型是用 OVA 致敏的。用 OVA 进行鼻内攻击后，通过 Buxco 系统、Liu 染色的支气管肺泡灌洗分析和酶联免疫吸附试验来确定气道炎症和高反应性。通过苏木精和伊红染色和免疫组织化学染色检测肺组织的组织病理学变化。
LGG 的预治疗和后治疗均抑制了对乙酰甲胆碱的气道高反应性，并与 OVA 致敏的小鼠相比，明显减少了支气管肺泡灌洗液和血清中的浸润性炎症细胞和 Th2 细胞因子的数量。此外，LGG 降低了血清中的 OVA 特异性 IgE 水平。口服 LGG 降低了肺组织中基质金属蛋白酶 9 的表达，并抑制了炎症细胞浸润。
LGG 对 OVA 诱导的气道炎症具有抗炎作用，可能是过敏性气道疾病的一种额外或补充治疗方法。
