Johansson M E, Brundin M, Adamson L, Grillner L, Landqvist M, Thörner A, Wirgart B Z
Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
J Med Virol. 1989 Jun;28(2):63-8. doi: 10.1002/jmv.1890280202.
The authors isolated during 1987 seven adenovirus type 31 (Ad31) within a 9-month period. The isolates were obtained from urine, throat, and feces, implying a systemic spread of the infection. Most patients displayed gastrointestinal symptoms, but some had respiratory symptoms and fever. All of the strains differed by restriction endonuclease analysis from the prototype strain (1315) by an additional Bgl II restriction site. Ad31 isolates 1-6 could be divided into two groups by the enzymes Bam HI, Msp I, and Xho I. Each enzyme gave rise to the same group distribution: isolates 1-3 and 4-6, respectively. Digestion with Bst EII, Hind III, Kpn I, and Sma I resulted in identical patterns for isolates 1-6. Isolate 7, however, demonstrated a DNA deletion of approximately 0.8 kbp, but it was otherwise identical to isolates 4-6. In conclusion, two separate genome types of Ad31 were isolated, one of which included a DNA deletion mutant. The increased isolation rate may reflect an epidemiological situation, as the same isolation procedure had been used both before and after this period.
1987年期间，作者在9个月内分离出7株31型腺病毒（Ad31）。这些分离株分别从尿液、咽喉和粪便中获得，这意味着感染存在全身扩散。大多数患者表现出胃肠道症状，但也有一些出现呼吸道症状和发热。通过限制性内切酶分析，所有菌株与原型菌株（1315）相比，均有一个额外的Bgl II限制性位点。Ad31分离株1 - 6可通过Bam HI、Msp I和Xho I酶分为两组。每种酶产生的分组分布相同：分别为分离株1 - 3和4 - 6。用Bst EII、Hind III、Kpn I和Sma I进行消化后，分离株1 - 6呈现相同的图谱。然而，分离株7显示出约0.8 kbp的DNA缺失，但在其他方面与分离株4 - 6相同。总之，分离出了两种不同的Ad31基因组类型，其中一种包括DNA缺失突变体。分离率的增加可能反映了一种流行病学情况，因为在此期间前后使用的是相同的分离程序。
