Kidd A H, Berkowitz F E, Blaskovic P J, Schoub B D
J Med Virol. 1984;14(3):235-46. doi: 10.1002/jmv.1890140307.
Twenty-six strains of faecal adenovirus from three continents (two from Europe, 16 from South Africa, and eight from Canada) were typed as Ad40 by restriction enzyme analysis of the DNA using Sma I. The DNA of the strains was further compared by digestion with six other enzymes, namely Bam HI, Eco RI, Bgl I, Xho I, Sst I, and Hind III. At least two different restriction profiles were found to exist for each enzyme. Nineteen of the 26 strains studied had the same combination of profiles for all the enzymes, within the limits of resolution of the technique. This genome type was detected from all three continents and included the Finnish reference strain Hovi-X. The other seven strains were genome variants, representing five other genome types. Three genome types were detected only in South African specimens, whereas one genome type was detected in single specimens from both South Africa and Canada. Strain Dugan from Holland, indistinguishable from other Ad40 strains in neutralization tests using polyclonal antibody preparations [de Jong et al, 1983], deviated from the common Hovi-X-like genome on digestion of the DNA with any of the enzymes except Sma I.
通过使用Sma I对DNA进行限制性酶切分析，对来自三大洲的26株粪便腺病毒（2株来自欧洲，16株来自南非，8株来自加拿大）进行了Ad40分型。通过用其他六种酶（即Bam HI、Eco RI、Bgl I、Xho I、Sst I和Hind III）消化，进一步比较了这些菌株的DNA。发现每种酶至少存在两种不同的限制性图谱。在所研究的26株菌株中，有19株在该技术的分辨率范围内，所有酶的图谱组合相同。这种基因组类型在三大洲均被检测到，包括芬兰参考菌株Hovi-X。其他七株菌株是基因组变体，代表其他五种基因组类型。三种基因组类型仅在南非标本中检测到，而一种基因组类型在来自南非和加拿大的单个标本中检测到。来自荷兰的Dugan菌株，在使用多克隆抗体制剂进行的中和试验中与其他Ad40菌株无法区分[de Jong等人，1983]，在用除Sma I之外的任何一种酶消化DNA时，偏离了常见的Hovi-X样基因组。
