Tsunemitsu H, Shimizu M, Hirai T, Yonemichi H, Kudo T, Mori K, Onoe S
Nihon Juigaku Zasshi. 1989 Apr;51(2):300-8. doi: 10.1292/jvms1939.51.300.
Three pregnant cows were inoculated intramuscularly with inactivated vaccine to bovine rotavirus (BRV) serotype 1 (BRV-1) and serotype 2 (BRV-2). Serum neutralizing antibody (NA) titers against both serotypes increased significantly after immunization. NA titers of colostrum obtained from immunized cows against BRV-1 and BRV-2 were 29286 and 38109, respectively, which were significantly higher than those from non-immunized control cows. Nine and 6 colostrum deprived calves were orally challenged with BRV-1 and BRV-2, respectively, and monitored for clinical manifestation and viral shedding. Five calves of them, 3 with BRV-1 and 2 with BRV-2, received 2 l of milk replacer supplemented with 10% immune colostrum 2 hr before challenge and twice daily for the first 5 days after challenge. Other 10 calves, 6 with BRV-1 and 4 with BRV-2, were fed only milk replacer as controls. All control calves developed severe diarrhea and shed a large amount of BRV in feces, beginning from 24 to 48 hr after challenge inoculation. On the contrary, all calves but one fed colostrum supplement remained clinically healthy after challenge, and BRV was not detected in their feces during feeding immune colostrum. The possibility that continuous feeding of immune colostrum is capable of preventing newborn calves from diarrhea associated with BRV and viral shedding was suggested.
给三头怀孕母牛肌肉注射了针对牛轮状病毒1型（BRV - 1）和2型（BRV - 2）的灭活疫苗。免疫后，针对这两种血清型的血清中和抗体（NA）滴度显著升高。从免疫母牛获得的初乳针对BRV - 1和BRV - 2的NA滴度分别为29286和38109，显著高于未免疫对照母牛的NA滴度。分别用BRV - 1和BRV - 2对9头和6头初乳缺乏的犊牛进行口服攻毒，并监测其临床表现和病毒排出情况。其中5头犊牛，3头用BRV - 1攻毒，2头用BRV - 2攻毒，在攻毒前2小时接受2升补充了10%免疫初乳的代乳品，并在攻毒后的前5天每天喂两次。另外10头犊牛，6头用BRV - 1攻毒，4头用BRV - 2攻毒，仅喂代乳品作为对照。所有对照犊牛从攻毒接种后24至48小时开始出现严重腹泻，并在粪便中排出大量BRV。相反，除一头犊牛外，所有喂食补充初乳的犊牛攻毒后保持临床健康，在喂食免疫初乳期间粪便中未检测到BRV。这表明持续喂食免疫初乳能够预防新生犊牛与BRV相关的腹泻和病毒排出。
