罗马尼亚东部地区猪和人类感染戊型肝炎的证据。
Evidence of hepatitis E infection in swine and humans in the East Region of Romania.
作者信息
Aniţă Adriana, Gorgan Lucian, Aniţă Dragoş, Oşlobanu Luanda, Pavio Nicole, Savuţa Gheorghe
机构信息
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 8 Mihail Sadoveanu Alley, 700489 Iaşi, Romania.
Faculty of Biology, Alexandru Ioan Cuza University, Iaşi, Romania.
出版信息
Int J Infect Dis. 2014 Dec;29:232-7. doi: 10.1016/j.ijid.2014.10.018. Epub 2014 Nov 5.
OBJECTIVE
Swine hepatitis E virus (HEV) is considered to be a new zoonotic agent due to its close genomic resemblance to the human HEV. The aim of this study was to determine human HEV seroprevalence in eastern Romania and to characterize circulating swine HEV sequences.
METHODS
Serological investigations of human serum samples were done using a commercial ELISA kit (MP Biomedicals). Swine faecal samples were tested to detect the HEV ORF2 sequence by nested reverse transcription PCR.
RESULTS
One hundred and forty-eight human serum samples were tested for anti-HEV IgG of which 22 were found to be positive. Fresh swine faeces (pools) were collected from five farms in eastern Romania. Six out of 19 pooled samples were positive for HEV RNA. Phylogenetic analysis based on alignment of the ORF2 sequence indicated that the Romanian swine HEV isolates belonged to genotype 3.
CONCLUSIONS
This is the first study showing HEV to be present in Romanian pig herds and that the human population is exposed.
目的
猪戊型肝炎病毒（HEV）因其基因组与人类HEV高度相似，被认为是一种新的人畜共患病原体。本研究旨在确定罗马尼亚东部地区人类HEV血清流行率，并对循环的猪HEV序列进行特征分析。
方法
使用商用ELISA试剂盒（MP Biomedicals）对人类血清样本进行血清学检测。通过巢式逆转录PCR检测猪粪便样本中的HEV ORF2序列。
结果
对148份人类血清样本进行抗HEV IgG检测，其中22份呈阳性。从罗马尼亚东部的五个农场收集了新鲜猪粪便（混合样本）。19份混合样本中有6份HEV RNA呈阳性。基于ORF2序列比对的系统发育分析表明，罗马尼亚猪HEV分离株属于3型。
结论
这是第一项表明罗马尼亚猪群中存在HEV且人群暴露于该病毒的研究。
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