来自长节珠根的微量细胞毒性强心苷类化合物。

Minor cytotoxic cardenolide glycosides from the root of Streptocaulon juventas.

作者信息

Ye Chun, Wang Hua, Xue Rui, Han Na, Wang Lihui, Yang Jingyu, Wang Yu, Yin Jun

机构信息

Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Steroids. 2015 Jan;93:39-46. doi: 10.1016/j.steroids.2014.10.005. Epub 2014 Nov 18.

DOI:10.1016/j.steroids.2014.10.005

PMID:25449765

Abstract

In order to determine new minor natural cardenolide glycosides as cytotoxic candidates, we isolated six new cardenolide glycosides together with four known ones, which had never previously been reported in the genus, by bioassay-guided separation from the 75% ethanol extract of Streptocaulon juventas (Asclepiadaceae). Their structures were elucidated on the basis of spectroscopic analysis, including homo- and heteronuclear correlation NMR experiments (COSY, HSQC and HMBC). The cytotoxic activities of these compounds were evaluated against A549 and NCI-H460 cell lines by MTT assay and compound 7 exhibited inhibitory activity against the two cell lines, while other compounds displayed a range of inhibitory activity against NCI-H460 and A549 cells. Their structure-activity relationships were also discussed.

摘要

为了确定新的具有细胞毒性的次要天然强心苷类化合物，我们通过生物活性导向分离法，从马利筋科植物长节链珠藤（Streptocaulon juventas）的75%乙醇提取物中分离出6种新的强心苷类化合物以及4种已知化合物，这些化合物在该属中此前从未有过报道。通过包括同核和异核相关NMR实验（COSY、HSQC和HMBC）在内的光谱分析确定了它们的结构。采用MTT法评估了这些化合物对A549和NCI-H460细胞系的细胞毒性活性，化合物7对这两种细胞系均表现出抑制活性，而其他化合物对NCI-H460和A549细胞则表现出一系列抑制活性。还讨论了它们的构效关系。