Kämpfer Peter, Trček Janja, Skok Barbara, Šorgo Andrej, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany,
Antonie Van Leeuwenhoek. 2015 Jun;107(6):1633-8. doi: 10.1007/s10482-015-0434-2. Epub 2015 Mar 27.
An intense yellow pigmented strain (SUR2(T)) isolated from dehydrated activated sludge was studied in detail to clarify its taxonomic assignment. Cells of the isolate showed a rod-shaped morphology and stained Gram-negative. Comparative 16S rRNA gene sequence analysis revealed highest similarities to the type strains of Chryseobacterium polytrichastri YG4-6(T) (98.6 %), Chryseobacterium aahli T68F(T) (97.9 %), Chryseobacterium daeguense K105(T) and Chryseobacterium gregarium DSM 79109(T) (both 97.4 %). 16S rRNA gene-sequence similarities to all other Chryseobacterium species were below 97.3 %. The fatty acid analysis of strain SUR2(T) revealed a Chryseobacterium typical profile composed mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA-DNA hybridizations with the type strains of C. polytrichastri, C. aahli, C. daeguense and C. gregarium resulted in values below 70 %. Differentiating biochemical and chemotaxonomic properties showed differences to the most closely related species and suggest that the isolate SUR2(T) represents a novel species, for which the name Chryseobacterium limigenitum sp. nov. (type strain SUR2(T) = ZIM B1019(T) = CCM 8594(T) = LMG 28734(T)) is proposed.
对从脱水活性污泥中分离出的一株强烈黄色色素菌株（SUR2(T)）进行了详细研究，以明确其分类地位。该分离株的细胞呈杆状形态，革兰氏染色阴性。16S rRNA基因序列比较分析显示，它与多毛发金黄杆菌YG4-6(T)（98.6%）、阿氏金黄杆菌T68F(T)（97.9%）、大邱金黄杆菌K105(T)和群居金黄杆菌DSM 79109(T)（均为97.4%）的模式菌株相似度最高。与所有其他金黄杆菌属物种的16S rRNA基因序列相似度均低于97.3%。菌株SUR2(T)的脂肪酸分析显示出典型的金黄杆菌属特征，主要由脂肪酸C15:0 iso、C15:0 iso 2-OH、C17:1 iso ω9c和C17:0 iso 3-OH组成。与多毛发金黄杆菌、阿氏金黄杆菌、大邱金黄杆菌和群居金黄杆菌的模式菌株进行DNA-DNA杂交，结果值低于70%。区分性的生化和化学分类学特性显示出与最密切相关物种的差异，表明分离株SUR2(T)代表一个新物种，为此提出名为Chryseobacterium limigenitum sp. nov.（模式菌株SUR2(T) = ZIM B1019(T) = CCM 8594(T) = LMG 28734(T)）。
