Hernández-Castellano Lorenzo E, Argüello Anastasio, Almeida André M, Castro Noemí, Bendixen Emøke
Department of Animal Science, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain; Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, CH-3001 Bern, Switzerland.
Department of Animal Science, Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Gran Canaria, Spain.
J Dairy Sci. 2015 Jan;98(1):135-47. doi: 10.3168/jds.2014-8143. Epub 2014 Nov 7.
Colostrum intake is a key factor for newborn ruminant survival because the placenta does not allow the transfer of immune components. Therefore, newborn ruminants depend entirely on passive immunity transfer from the mother to the neonate, through the suckling of colostrum. Understanding the importance of specific colostrum proteins has gained significant attention in recent years. However, proteomics studies of sheep colostrum and their uptake in neonate lambs has not yet been presented. The aim of this study was to describe the proteomes of sheep colostrum and lamb blood plasma, using sodium dodecyl sulfate-PAGE for protein separation and in-gel digestion, followed by liquid chromatography-tandem mass spectrometry of resulting tryptic peptides for protein identification. An isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was subsequently used to provide relative quantification of how neonatal plasma protein concentrations change as an effect of colostrum intake. The results of this study describe the presence of 70 proteins in the ovine colostrum proteome. Furthermore, colostrum intake resulted in an increase of 8 proteins with important immune functions in the blood plasma of lambs. Further proteomic studies will be necessary, particularly using the selected reaction monitoring approach, to describe in detail the role of specific colostrum proteins for immune transfer to the neonate.
初乳摄入是新生反刍动物存活的关键因素，因为胎盘不允许免疫成分的传递。因此，新生反刍动物完全依赖于通过初乳哺乳从母体向新生儿的被动免疫传递。近年来，了解特定初乳蛋白的重要性受到了广泛关注。然而，尚未有关于绵羊初乳及其在新生羔羊体内摄取的蛋白质组学研究报道。本研究的目的是描述绵羊初乳和羔羊血浆的蛋白质组，使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行蛋白质分离和胶内消化，随后通过液相色谱-串联质谱法对所得胰蛋白酶肽段进行蛋白质鉴定。随后采用基于相对和绝对定量的等压标签（iTRAQ）蛋白质组学方法，对初乳摄入对新生儿血浆蛋白浓度变化的影响进行相对定量。本研究结果描述了绵羊初乳蛋白质组中存在70种蛋白质。此外，初乳摄入导致羔羊血浆中8种具有重要免疫功能的蛋白质增加。进一步的蛋白质组学研究是必要的，特别是使用选择反应监测方法，以详细描述特定初乳蛋白在向新生儿免疫转移中的作用。
