蛋白质组学驱动的绵羊初乳乳清分析。

Scumaci Domenica, Trimboli Francesca, Dell'Aquila Ludovica, Concolino Antonio, Pappaianni Giusi, Tammè Laura, Vignola Giorgio, Luciani Alessia, Morelli Daniela, Cuda Giovanni, Boari Andrea, Britti Domenico

Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.

Dpt. of Health Science, Magna Græcia University of Catanzaro, Catanzaro, Italy.

PLoS One. 2015 Feb 2;10(2):e0117433. doi: 10.1371/journal.pone.0117433. eCollection 2015.

The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.

本研究的目的是深入了解绵羊初乳蛋白质组的复杂性，特别关注低丰度蛋白质。绵羊初乳的特点是有几种占主导地位的蛋白质，如免疫球蛋白，但它也含有较少的蛋白质种类，这些对新生儿的正常发育同样重要。产羔后立即采集的绵羊初乳通过一维SDS-PAGE进行分离。蛋白质条带用胰蛋白酶消化，所得肽段通过LC-MS/MS进行分析。基于Swiss-Prot数据库，共鉴定出343种独特的蛋白质。据我们所知，本研究是对绵羊初乳蛋白质组最全面的分析。