Palaniappan P, Sathishkumar G, Sankar R
Department of Environmental Science and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea; Department of Biochemistry, J.J. College of Arts and Science (Autonomous), Pudukkottai 622 422, Tamilnadu, India.
Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Mar 5;138:885-90. doi: 10.1016/j.saa.2014.10.072. Epub 2014 Nov 6.
The present study reports, green synthesis of bioactive silver nanoparticles (AgNPs) under different temperature (60°C, room temperature and 4° refrigerator) using the aqueous extract of sea grass Cymodocea serrulata as a potential bioreductant. Increased temperature fabricates more AgNPs compare to room temperature and refrigerator condition. At first the reduction of Ag(+) ions were confirmed through color change which produces an absorbance spectra at 420nm in UV-Visible spectrophotometer. Additionally various exclusive instrumentations such as X-ray diffraction (XRD), Dynamic light scattering (DLS), scanning electron microscope (SEM) analysis and Transmission electron microscope (TEM) were authorizes the biosynthesis and physio-chemical characterization of AgNPs. From Fourier transform infrared spectroscopy (FTIR) analysis, it was identified that the water soluble fractions of the sea grass mainly responsible for reduction of ionic silver (Ag(+)) into (Ag(0)) nano-ranged particles and also they act as stabilizing agent to sustain the durability of NPs for long period of time. Further, synthesized AgNPs shows potential cytotoxicity against human lung cancer A549 cells (LD50-100μg/ml). The overall results suggest that C. serrulata is a valuable bioresource to generate rapid and eco-friendly bioactive AgNPs towards cancer therapy.
本研究报告了以锯齿泰来草的水提取物作为潜在生物还原剂，在不同温度（60°C、室温及4°C冰箱）下生物合成具有生物活性的银纳米颗粒（AgNPs）。与室温及冰箱条件相比，升高温度可制备出更多的AgNPs。首先，通过颜色变化证实了Ag(+)离子的还原，该颜色变化在紫外可见分光光度计中产生420nm处的吸收光谱。此外，诸如X射线衍射（XRD）、动态光散射（DLS）、扫描电子显微镜（SEM）分析及透射电子显微镜（TEM）等各种专用仪器证实了AgNPs的生物合成及理化特性。通过傅里叶变换红外光谱（FTIR）分析确定，锯齿泰来草的水溶性部分主要负责将离子银（Ag(+)）还原为纳米级颗粒（Ag(0)），并且它们还作为稳定剂长期维持纳米颗粒的稳定性。此外，合成的AgNPs对人肺癌A549细胞显示出潜在的细胞毒性（半数致死剂量为100μg/ml）。总体结果表明，锯齿泰来草是一种宝贵的生物资源，可用于快速、环保地生成具有生物活性的AgNPs用于癌症治疗。
