Neal-McKinney Jason M, Samuelson Derrick R, Eucker Tyson P, Nissen Mark S, Crespo Rocio, Konkel Michael E
School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America.
Avian Health & Food Safety Laboratory, College of Veterinary Medicine, Washington State University, Puyallup, Washington, United States of America.
PLoS One. 2014 Dec 4;9(12):e114254. doi: 10.1371/journal.pone.0114254. eCollection 2014.
Campylobacter jejuni is a leading bacterial cause of human gastrointestinal disease worldwide. While C. jejuni is a commensal organism in chickens, case-studies have demonstrated a link between infection with C. jejuni and the consumption of foods that have been cross-contaminated with raw or undercooked poultry. We hypothesized that vaccination of chickens with C. jejuni surface-exposed colonization proteins (SECPs) would reduce the ability of C. jejuni to colonize chickens, thereby reducing the contamination of poultry products at the retail level and potentially providing a safer food product for consumers. To test our hypothesis, we injected chickens with recombinant C. jejuni peptides from CadF, FlaA, FlpA, CmeC, and a CadF-FlaA-FlpA fusion protein. Seven days following challenge, chickens were necropsied and cecal contents were serially diluted and plated to determine the number of C. jejuni per gram of material. The sera from the chickens were also analyzed to determine the concentration and specificity of antibodies reactive against the C. jejuni SECPs. Vaccination of chickens with the CadF, FlaA, and FlpA peptides resulted in a reduction in the number of C. jejuni in the ceca compared to the non-vaccinated C. jejuni-challenged group. The greatest reduction in C. jejuni colonization was observed in chickens injected with the FlaA, FlpA, or CadF-FlaA-FlpA fusion proteins. Vaccination of chickens with different SECPs resulted in the production of C. jejuni-specific IgY antibodies. In summary, we show that the vaccination of poultry with individual C. jejuni SECPs or a combination of SECPs provides protection of chickens from C. jejuni colonization.
空肠弯曲菌是全球人类胃肠道疾病的主要细菌性病因。虽然空肠弯曲菌是鸡体内的共生菌，但案例研究表明，感染空肠弯曲菌与食用被生的或未煮熟的家禽交叉污染的食物之间存在联系。我们假设用空肠弯曲菌表面暴露的定植蛋白（SECPs）对鸡进行疫苗接种会降低空肠弯曲菌在鸡体内定植的能力，从而减少零售层面家禽产品的污染，并有可能为消费者提供更安全的食品。为了验证我们的假设，我们给鸡注射了来自CadF、FlaA、FlpA、CmeC的重组空肠弯曲菌肽以及一种CadF-FlaA-FlpA融合蛋白。攻毒7天后，对鸡进行剖检，将盲肠内容物进行系列稀释并接种平板，以确定每克材料中空肠弯曲菌的数量。还对鸡的血清进行了分析，以确定针对空肠弯曲菌SECPs的反应性抗体的浓度和特异性。与未接种疫苗的空肠弯曲菌攻毒组相比，用CadF、FlaA和FlpA肽对鸡进行疫苗接种导致盲肠中空肠弯曲菌数量减少。在注射FlaA、FlpA或CadF-FlaA-FlpA融合蛋白的鸡中，观察到空肠弯曲菌定植减少最为明显。用不同的SECPs对鸡进行疫苗接种导致产生了空肠弯曲菌特异性IgY抗体。总之，我们表明用单个空肠弯曲菌SECPs或SECPs组合对家禽进行疫苗接种可保护鸡免受空肠弯曲菌定植。
