Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Innovative Technology Research Institute of Tissue Repair and Regeneration, Key Laboratory of Regenerative Medicine, Ministry of Education, Guangzhou Guangdong Province 510630, China.
Department of Plastic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China.
Biomed Res Int. 2020 Dec 21;2020:6406395. doi: 10.1155/2020/6406395. eCollection 2020.
Adipose-derived stem cells (ADSCs) have been documented as possible candidates for skin rejuvenation. However, the effects of ADSC-derived exosomes on photoaged skin remain to be fully elucidated. This study was aimed at determining the antiaging effects of ADSC-derived exosomes on photoaged skin. Human ADSCs were isolated from the adipose tissue of healthy women and cultured in vitro. Then, exosomes were extracted from the cultured ADSCs, purified by ultracentrifugation, and verified by examination of cell morphology using transmission electron microscopy and the identification of specific biomarkers. Meanwhile, the optimal exosome concentration and treatment time were selected. The photoaged skin model was created by subjecting Sprague-Dawley rats to ultraviolet B radiation. Exosomes were injected into the photoaged skin in a single therapeutic dose. The thickness of the epidermis and dermis was observed by HE staining. The relative mRNA expression of type I collagen, type III collagen, and matrix metalloproteinases (MMP-1 and MMP-3) was determined by real-time PCR. In the rat model of photoaged skin, the injected exosomes markedly decreased the epidermal thickness and increased the dermal thickness of the photoaged skin 7 days after treatment. Moreover, the proportion of the stratum corneum of the epidermis was decreased. Furthermore, real-time RT-PCR showed that the mRNA expression of type I collagen was increased and that of type III collagen, MMP-1, and MMP-3 was decreased. Our results demonstrate that ADSC-derived exosome treatment could significantly improve skin photodamage and that ADSC-derived exosomes may be a potential agent for photoaged skin treatment.
脂肪来源干细胞 (ADSCs) 已被证明是皮肤年轻化的潜在候选者。然而，ADSC 衍生的外泌体对光老化皮肤的影响仍有待充分阐明。本研究旨在确定 ADSC 衍生的外泌体对光老化皮肤的抗衰老作用。从健康女性的脂肪组织中分离出人 ADSC 并在体外培养。然后，从培养的 ADSC 中提取外泌体，通过超速离心进行纯化，并通过透射电子显微镜检查细胞形态和鉴定特定生物标志物来验证。同时，选择最佳的外泌体浓度和治疗时间。通过对 Sprague-Dawley 大鼠进行紫外线 B 辐射来创建光老化皮肤模型。将外泌体单次治疗剂量注入光老化皮肤中。通过 HE 染色观察表皮和真皮的厚度。通过实时 PCR 测定 I 型胶原、III 型胶原和基质金属蛋白酶 (MMP-1 和 MMP-3) 的相对 mRNA 表达。在光老化皮肤大鼠模型中，注射的外泌体在治疗后 7 天明显降低了光老化皮肤的表皮厚度并增加了真皮厚度。此外，表皮的角质层比例降低。此外，实时 RT-PCR 显示 I 型胶原的 mRNA 表达增加，而 III 型胶原、MMP-1 和 MMP-3 的表达减少。我们的结果表明，ADSC 衍生的外泌体治疗可显著改善皮肤光损伤，ADSC 衍生的外泌体可能是治疗光老化皮肤的潜在药物。
