Ghildyal N, Schnitzlein W M, Tripathy D N
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana.
Arch Virol. 1989;106(1-2):85-92. doi: 10.1007/BF01311040.
The genomes of a fowlpox and quailpox virus isolate were compared by restriction enzyme analysis using BamHI, EcoRI, and HindIII endonucleases. The genetic profiles of the two virus species were very distinct with fragments lacking similar electrophoretic mobilities. In contrast, the patterns of three quailpox virus isolates were very similar with a high proportion of co-migrating fragments. When the immunogenic proteins of two quailpox, three fowlpox, a juncopox, and a pigeonpox virus isolate were examined by immunoblotting, common as well as unique antigens were detected. The greatest disparity was between quailpox virus and the other three species which are genomically conserved. Therefore, on the basis of genetic as well as immunological analysis, quailpox virus is a distinct species of the genus Avipoxvirus.
利用BamHI、EcoRI和HindIII核酸内切酶，通过限制性酶切分析比较了一株禽痘病毒和一株鹌鹑痘病毒的基因组。这两种病毒的遗传图谱非常不同，片段缺乏相似的电泳迁移率。相比之下，三株鹌鹑痘病毒分离株的图谱非常相似，共迁移片段比例很高。当通过免疫印迹法检测两株鹌鹑痘病毒、三株禽痘病毒、一株朱顶雀痘病毒和一株鸽痘病毒分离株的免疫原性蛋白时，检测到了共同抗原和独特抗原。鹌鹑痘病毒与其他三个基因组保守的物种之间差异最大。因此，基于遗传和免疫学分析，鹌鹑痘病毒是禽痘病毒属的一个独特物种。
