牛肾上腺皮质微粒体的肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）结合位点：功能与调节
The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation.
作者信息
Palmer S, Wakelam M J
机构信息
Molecular Pharmacology Group, Institute of Biochemistry, University of Glasgow, U.K.
出版信息
Biochem J. 1989 Jun 1;260(2):593-6. doi: 10.1042/bj2600593.
Abstract
Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.
摘要
肾上腺皮质微粒体具有单一群体的肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）特异性结合位点[半数抑制浓度（IC50）为5.9±0.9纳摩尔；帕尔默、休斯、李和韦克莱姆（1988年）《细胞信号传导》1, 147 - 156]。竞争研究表明，肌醇-1,2-环化,4,5-三磷酸（Ins(1:2-cyclic,4,5)P3）对该位点的亲和力比Ins(1,4,5)P3低21倍（IC50为124±16纳摩尔）。Ins(1,4,5)P3结合位点对Ins(1,4,5)P3的亲和力不受不可水解的鸟苷三磷酸（GTP）类似物鸟苷-5'-O-(3-硫代三磷酸)（GTPγS）和鸟苷-5'-O-(2-氨基)三磷酸（Gpp[NH]p）的影响，也不受在三磷酸腺苷（ATP）和12-O-十四酰佛波醇-13-乙酸酯（TPA）存在下，将结合蛋白与部分纯化的蛋白激酶C制剂预孵育的影响。结合肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）结合位点的特性和功能对这些观察结果进行了讨论。
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本文引用的文献
1
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Biochem J. 1984 Dec 15;224(3):741-6. doi: 10.1042/bj2240741.
2
Phorbol diester receptor copurifies with protein kinase C.佛波酯受体与蛋白激酶C共纯化。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):36-40. doi: 10.1073/pnas.80.1.36.
3
Inositol trisphosphate and diacylglycerol as second messengers.肌醇三磷酸和二酰甘油作为第二信使。
Biochem J. 1984 Jun 1;220(2):345-60. doi: 10.1042/bj2200345.
4
Inositol trisphosphate, a novel second messenger in cellular signal transduction.肌醇三磷酸，细胞信号转导中的一种新型第二信使。
Nature. 1984;312(5992):315-21. doi: 10.1038/312315a0.
5
Muscarinic receptor-mediated increase in cytoplasmic free Ca2+ in isolated bovine adrenal medullary cells. Effects of TMB-8 and phorbol ester TPA.毒蕈碱受体介导的分离牛肾上腺髓质细胞胞质游离钙离子增加。丁基苯酞-8（TMB-8）和佛波酯十四酰佛波醇乙酯（TPA）的作用
FEBS Lett. 1985 Oct 7;190(1):25-8. doi: 10.1016/0014-5793(85)80419-1.
6
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