Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China.
Bioorg Med Chem Lett. 2013 Oct 15;23(20):5714-20. doi: 10.1016/j.bmcl.2013.08.006. Epub 2013 Aug 11.
Investigation of the n-BuOH extract of the rhizomes of Anemone taipaiensis led to the isolation of five new oleanane-type triterpenoid saponins (1-5), together with seven known saponins (6-12). Their structures were determined by the extensive use of (1)D and (2)D NMR experiments along with ESIMS analyses and acid hydrolysis. The aglycone of 1, 2 and 4 was determined as siaresinolic acid, which was reported in this genus for the first time. The cytotoxicities of the saponins 1-12, prosapogenins 4a, 5a, 10a-12a and sapogenins siaresinolic acid (SA), oleanolic acid (OA), hederagenin (HE) were evaluated against five human cancer cell lines, including HepG2, HL-60, A549, HeLa and U87MG. The monodesmosidic saponins 6-8, 5a, 10a-12a and sapogenins SA, OA, HE exhibited cytotoxic activity toward all cancer cell lines, with IC50 values ranging from 2.25 to 57.28 μM. Remarkably, the bisdesmosidic saponins 1-4 and 9 showed selective cytotoxicity against the U87MG cells.
对白头翁根茎的正丁醇提取物进行研究，分离得到了五个新的齐墩果烷型三萜皂苷（1-5），以及七个已知的皂苷（6-12）。通过广泛使用（1）D 和（2）D NMR 实验以及 ESIMS 分析和酸水解，确定了它们的结构。1、2 和 4 的糖苷配基被确定为齐墩果酸，这是该属中首次报道。对皂苷 1-12、原皂苷 4a、5a、10a-12a 和皂苷元齐墩果酸（SA）、熊果酸（OA）、羽扇豆烷（HE）进行了评估，对五种人癌细胞系（包括 HepG2、HL-60、A549、HeLa 和 U87MG）进行了评估。单糖苷皂苷 6-8、5a、10a-12a 和皂苷元 SA、OA、HE 对所有癌细胞系均表现出细胞毒性，IC50 值范围为 2.25 至 57.28 μM。值得注意的是，双糖苷皂苷 1-4 和 9 对 U87MG 细胞表现出选择性细胞毒性。
