Department of Clinical Dentistry, Periodontics, University of Bergen, Bergen, Norway.
Department of Applied Oral Sciences, Center for Periodontology, The Forsyth Institute, Cambridge, MA, 02142, USA.
J Periodontal Res. 2015 Oct;50(5):674-82. doi: 10.1111/jre.12250. Epub 2014 Dec 9.
Aggressive periodontitis (AgP) is prevalent and shows a rapid course in African individuals. Although a strong focus has been placed on Aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in AgP. The purpose of the present study was to map the subgingival microbiota as well as explore the presence of A. actinomycetemcomitans and the JP2 clone in a group of Sudanese individuals with AgP, using different analytical methods.
A study population consisting of 19 patients with AgP was recruited from patients seeking treatment at University of Science and Technology (UST) in Khartoum. Fifteen healthy subjects were included as controls. Plaque samples were analyzed for 272 taxa using human oral microbe identification microarrays and for 26 periodontal taxa using DNA-DNA hybridization checkerboard. Conventional polymerase chain reaction (PCR) was applied for the detection of A. actinomycetemcomitans and the JP2 clone in plaque. Saliva from patients with AgP was analyzed using quantitative PCR (qPCR) for the detection of A. actinomycetemcomitans.
Eubacterium yurii was detected more frequently in patients with AgP than in controls, and E. nodatum was found in patients with AgP only. A. actinomycetemcomitans was found in plaque samples of two (12%) patients by human oral microbe identification microarrays and in five (29%) patients with AgP by conventional PCR, as well as in six (32%) of the AgP saliva samples by qPCR. The JP2 clone was identified in only one patient.
The classical periodontal pathogens were not present in high amounts in AgP in the population studied here. Species of Eubacterium, which are not typically associated with AgP, were often detected in individuals with disease. Using laboratory methods with different sensitivities and detection levels allowed identification of variances in microbial communities. The findings reported in this study provide a basis for the further understanding of AgP.
侵袭性牙周炎（AgP）在非洲人群中较为常见且呈快速发展趋势。虽然人们一直高度关注伴放线放线杆菌（Aggregatibacter actinomycetemcomitans），但新方法支持 AgP 中存在复杂的龈下微生物群。本研究旨在通过不同分析方法，对一组患有 AgP 的苏丹个体的龈下微生物群进行描绘，并探索伴放线放线杆菌和 JP2 克隆的存在情况。
从在喀土穆的 UST 寻求治疗的 AgP 患者中招募了由 19 名患者组成的研究人群，并纳入 15 名健康个体作为对照。使用人类口腔微生物识别微阵列分析了 272 个分类单元和 DNA-DNA 杂交 checkerboard 分析了 26 个牙周分类单元的菌斑样本。采用常规聚合酶链反应（PCR）检测菌斑中是否存在伴放线放线杆菌和 JP2 克隆。使用定量聚合酶链反应（qPCR）分析 AgP 患者的唾液中是否存在伴放线放线杆菌。
与对照组相比，患者的尤氏菌（Eubacterium yurii）检出率更高，而只有患者检出真杆菌（E. nodatum）。人类口腔微生物识别微阵列在两名（12%）患者的菌斑样本中检测到伴放线放线杆菌，在五名（29%）AgP 患者中通过常规 PCR 检测到伴放线放线杆菌，在六名（32%）AgP 唾液样本中通过 qPCR 检测到伴放线放线杆菌。仅在一名患者中鉴定出 JP2 克隆。
在本研究人群中，经典牙周病原体在 AgP 中并未大量存在。在患有疾病的个体中，通常检测到通常与 AgP 无关的尤氏菌属物种。使用具有不同敏感性和检测水平的实验室方法可以识别微生物群落的差异。本研究报告的结果为进一步了解 AgP 提供了基础。
