Section for Microbiology and Immunology, The Gade Institute, University of Bergen, Norway.
J Periodontal Res. 2011 Jun;46(3):285-91. doi: 10.1111/j.1600-0765.2010.01337.x. Epub 2011 Feb 17.
Aggregatibacter actinomycetemcomitans is considered a possible etiological agent for aggressive periodontitis. The aim of this study was to determine the prevalence of the JP2 clone and non-JP2 genotypes of A. actinomycetemcomitans in the subgingival plaque of patients with aggressive periodontitis and controls among Sudanese high-school students.
In a previous study we examined a large representative sample of students attending high schools in Khartoum, Sudan. In this population, 17 patients with aggressive periodontitis and 17 controls (14-19 years of age) consented to participate in the present study. The subjects underwent a clinical periodontal examination, and subgingival dental plaque samples were collected using paper points. The presence of the A. actinomycetemcomitans JP2 clone and non-JP2 genotypes were assessed using loop-mediated isothermal amplification (LAMP) and the PCR.
The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of either the cases or the controls. Non-JP2 types of A. actinomycetemcomitans were detected in the subgingival plaque of 12 (70.6%) patients with aggressive periodontitis and from only one (5.9%) control subject, showing a significantly higher frequency of detection in cases than in controls (p = 0.0001). The odds ratio for the detection of A. actinomycetemcomitans in the subgingival plaque of the patients with aggressive periodontitis was 38.4 (95% confidence interval: 4.0-373.0; p = 0.002). The PCR and LAMP methods showed identical results pertaining to the identification of non-JP2 types of A. actinomycetemcomitans.
The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of high school subjects in Sudan. The detection of non-JP2 types of A. actinomycetemcomitans may be a useful marker of increased risk for development of aggressive periodontitis in young subjects.
聚集放线杆菌被认为是侵袭性牙周炎的可能病因。本研究旨在确定苏丹高中生侵袭性牙周炎患者和对照者龈下菌斑中 JP2 克隆和非 JP2 基因型聚集放线杆菌的流行率。
在之前的一项研究中，我们检查了苏丹喀土穆一所高中的大量代表性学生样本。在该人群中，17 名侵袭性牙周炎患者和 17 名对照者（14-19 岁）同意参与本研究。受试者接受了临床牙周检查，并使用纸尖采集龈下牙菌斑样本。使用环介导等温扩增（LAMP）和 PCR 评估 A. actinomycetemcomitans JP2 克隆和非 JP2 基因型的存在。
在病例或对照者的龈下菌斑中均未检测到 A. actinomycetemcomitans JP2 克隆。在 12 名（70.6%）侵袭性牙周炎患者的龈下菌斑中检测到非 JP2 型 A. actinomycetemcomitans，而在 1 名（5.9%）对照者中仅检测到 1 名，病例组的检出频率明显高于对照组（p = 0.0001）。侵袭性牙周炎患者龈下菌斑中 A. actinomycetemcomitans 的检出率的优势比为 38.4（95%置信区间：4.0-373.0；p = 0.002）。PCR 和 LAMP 方法在鉴定非 JP2 型 A. actinomycetemcomitans 方面显示出相同的结果。
在苏丹的高中生龈下菌斑中未检测到 A. actinomycetemcomitans JP2 克隆。检测非 JP2 型 A. actinomycetemcomitans 可能是年轻受试者侵袭性牙周炎发展风险增加的有用标志物。
