Pardo-Barrela Jessica, Lago-Crespo Miguel, Lage-Yusty María Asunción, López-Hernández Julia
Analytical Chemistry, Nutrition and Bromatology Department Pharmacy Faculty, Campus Vida University of Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Plant Foods Hum Nutr. 2015 Mar;70(1):91-6. doi: 10.1007/s11130-014-0454-5.
Quercetin is a polyphenol of growing interest that is present in many foods. In this study, we compared two methods for its determination in samples of drinks made of juice and in dietary supplements, one chromatographic (HPLC) and other spectrofluorimetric (constant-wavelength synchronous spectrofluorimetry). To confirm the identification of the quercetin in the samples an HPLC-PDA-MS/MS system was used. It was concluded that both methods are suitable for dietary supplements and the choice of one or the other depends on the type of sample, time available for the analysis as well as the available resources. For juice beverages only HPLC is suitable.
槲皮素是一种越来越受关注的多酚类物质，存在于许多食物中。在本研究中，我们比较了两种测定果汁饮料样品和膳食补充剂中槲皮素的方法，一种是色谱法（高效液相色谱法），另一种是荧光光谱法（恒波长同步荧光光谱法）。为了确认样品中槲皮素的鉴定，使用了高效液相色谱-光电二极管阵列-串联质谱系统。得出的结论是，两种方法都适用于膳食补充剂，选择哪种方法取决于样品类型、可用于分析的时间以及可用资源。对于果汁饮料，仅高效液相色谱法适用。
