用于检测单纯疱疹病毒的空斑小瓶离心法的改良
Modification of shell vial centrifugation method for detection of herpes simplex virus.
作者信息
Lin S Y
机构信息
Department of Microbiology, Northern California Regional Laboratory, Kaiser Permanente Medical Care Program, Berkeley 94710.
出版信息
J Clin Microbiol. 1989 Aug;27(8):1896-7. doi: 10.1128/jcm.27.8.1896-1897.1989.
Abstract
The shell vial centrifugation method for the detection of herpes simplex virus was modified with the addition of maintenance medium before specimen inoculation rather than after. Sensitivity and specificity values were comparable to those obtained with postcentrifugation medium addition.
摘要
用于检测单纯疱疹病毒的空斑离心法进行了改进，即在接种标本前而非接种后添加维持培养基。敏感性和特异性值与在离心后添加培养基时所获得的值相当。
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本文引用的文献
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Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.接种后16小时，通过离心和单克隆抗体在MRC-5细胞中检测单纯疱疹病毒并进行血清分型。
J Clin Microbiol. 1985 Jan;21(1):29-32. doi: 10.1128/jcm.21.1.29-32.1985.
2
Evaluation of a shell vial centrifugation method for the detection of herpes simplex virus.
Diagn Microbiol Infect Dis. 1988 Jan;9(1):51-4. doi: 10.1016/0732-8893(88)90061-2.
3
Conventional tube cell culture compared with centrifugal inoculation of MRC-5 cells and staining with monoclonal antibodies for detection of herpes simplex virus in clinical specimens.传统的管细胞培养与MRC-5细胞的离心接种以及用单克隆抗体染色用于检测临床标本中的单纯疱疹病毒的比较。
J Clin Microbiol. 1988 Mar;26(3):570-2. doi: 10.1128/jcm.26.3.570-572.1988.
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