Woods G L, Mills R D
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.
J Clin Microbiol. 1988 Mar;26(3):570-2. doi: 10.1128/jcm.26.3.570-572.1988.
During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional tube cell culture with primary rabbit kidney and A549 cells. HSV was identified by conventional tube cell culture in 165 (24%) of 699 specimens and by the 24WPC method in 116 (17%) of 699 specimens. One specimen was positive for HSV by the 24WPC method alone, compared with 50 specimens positive only by conventional cell culture (P less than 0.0001). The sensitivity, specificity, and positive and negative predictive values of the 24WPC technique with MRC-5 cells for detection of HSV in clinical specimens were 70, 99.8, 99, and 91%, respectively. Centrifugal inoculation of MRC-5 cells in 24-well plates and staining with monoclonal antibodies after incubation for 16 to 18 h is an insensitive means of detecting HSV in clinical specimens and should not replace conventional tube cell culture with primary rabbit kidney cells.
在15个月的时间里，对699份临床标本中检测单纯疱疹病毒（HSV）的两种方法进行了比较：（i）将MRC-5细胞接种于24孔板进行离心（24WPC），孵育16至18小时后用型特异性单克隆抗体（Syva公司，加利福尼亚州帕洛阿尔托）染色；（ii）用原代兔肾细胞和A549细胞进行传统的试管细胞培养。在699份标本中，通过传统试管细胞培养法在165份（24%）标本中鉴定出HSV，通过24WPC法在699份标本中的116份（17%）中鉴定出HSV。仅24WPC法检测出1份标本HSV阳性，而仅通过传统细胞培养法检测出50份标本阳性（P<0.0001）。用MRC-5细胞的24WPC技术检测临床标本中HSV的敏感性、特异性、阳性预测值和阴性预测值分别为70%、99.8%、99%和91%。将MRC-5细胞接种于24孔板进行离心接种，孵育16至18小时后用单克隆抗体染色，是检测临床标本中HSV的一种不敏感方法，不应取代用原代兔肾细胞进行的传统试管细胞培养。
