Matsuda Y, Nakagomi O
Animal Facilities for Experimental Medicine, Akita University School of Medicine, Japan.
Res Virol. 1989 Jul-Aug;140(4):337-50. doi: 10.1016/s0923-2516(89)80114-1.
Japanese bovine rotavirus isolates, which fall into two different serotypes, were shown to belong to subgroup I and to have long RNA electrophoretypes. This study confirmed the distinction of two serotypes on the basis of a greater than 20-fold difference in neutralization titres between the homologous and heterologous reactions; however, significant one-way cross-neutralization was observed between one of the strains with bovine serotype 2 and antisera to strains with bovine serotype 1 (serotype 6 according to the unified serotyping system). When 32P-labelled transcription probes prepared from Japanese strains as well as a prototype NCDV strain were hybridized with genomic double-stranded RNA from these bovine strains and from prototype human strains, a high level of homology was observed among bovine rotavirus strains; but this level of homology was not found between bovine strains and any of the prototype human strains. These results suggest that bovine rotavirus strains belong to a single genogroup that is distinct from any of the human genogroups previously identified by RNA-RNA hybridization.
日本牛轮状病毒分离株分为两种不同血清型，属于I亚组，具有长RNA电泳型。本研究基于同源和异源反应之间中和滴度大于20倍的差异，证实了两种血清型的区别；然而，在一株牛血清型2毒株与牛血清型1（根据统一血清分型系统为血清型6）毒株的抗血清之间观察到了显著的单向交叉中和。当用日本毒株以及一株NCDV原型毒株制备的32P标记转录探针与这些牛毒株和原型人毒株的基因组双链RNA杂交时，在牛轮状病毒毒株之间观察到了高度同源性；但在牛毒株与任何原型人毒株之间未发现这种同源性水平。这些结果表明，牛轮状病毒毒株属于一个单一的基因群，与先前通过RNA-RNA杂交鉴定的任何人类基因群都不同。
