Liu Xiao-Yan, Wang Chang-Peng, Liu Ming, Ji Gang, Guo Jing-Chun
State Key Laboratory of Medical Neurobiology, Department of Neurobiology, School of Basic Medical Science, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Sheng Li Xue Bao. 2014 Dec 25;66(6):691-701.
The purpose of this study is to explore the fate and effect of human embryonic neural stem cells (hNSCs) after transplantation into ipsilateral lateral ventricle of stroke rats. Adult rats were exposed to one-hour transient middle cerebral artery occlusion (MCAO), and then hNSCs were transplanted into ipsilateral lateral ventricle 7 days after reperfusion. Infarct volume was calculated by cresyl violet staining. The improvements of neural functions were assessed by behavioral tests. Immunofluorescence staining was performed to observe the migration and differentiation of transplanted hNSCs. The results showed that transplanted hNSCs significantly reduced ischemia-induced infarction in MCAO rats, and improved neural functional restoration when assessed by rotarod, footfault and corner-turn tests. The grafted cells migrated predominantly to several specific brain regions, such as corpus callosum and peri-infarct area. Furthermore, these cells differentiated into oligodendrocytes and astrocytes in corpus callosum, and neurons in peri-infarct parenchyma. These results suggest that transplanted hNSCs through lateral ventricle of the ischemic side may exert effective therapeutic effects on stroke rats via migration and differentiation in specific brain regions.
本研究的目的是探讨人胚胎神经干细胞（hNSCs）移植到中风大鼠同侧侧脑室后的命运和作用。成年大鼠接受1小时短暂性大脑中动脉闭塞（MCAO），然后在再灌注7天后将hNSCs移植到同侧侧脑室。通过甲酚紫染色计算梗死体积。通过行为测试评估神经功能的改善情况。进行免疫荧光染色以观察移植的hNSCs的迁移和分化。结果表明，移植的hNSCs显著减少了MCAO大鼠缺血诱导的梗死，并在通过转棒试验、足错误试验和转角试验评估时改善了神经功能恢复。移植的细胞主要迁移到几个特定的脑区，如胼胝体和梗死周围区域。此外，这些细胞在胼胝体中分化为少突胶质细胞和星形胶质细胞，在梗死周围实质中分化为神经元。这些结果表明，通过缺血侧侧脑室移植的hNSCs可能通过在特定脑区的迁移和分化对中风大鼠发挥有效的治疗作用。
