Godara R, Katoch M, Katoch R, Yadav Anish, Parveen S, Vij Bhavna, Khajuria Varun, Singh G, Singh Nirbhay K
Division of Veterinary Parasitology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, R. S. Pura, Jammu 181 102, India.
Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180 001, India.
ScientificWorldJournal. 2014;2014:713170. doi: 10.1155/2014/713170. Epub 2014 Nov 12.
In vitro efficacy of methanolic extract of Atropa belladonna and its components scopolamine and atropine was assessed against Rhipicephalus (Boophilus) microplus. Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) were used whereas scopolamine and atropine were each tested at 0.1%. In adult immersion test, the extract was lethal to ticks at 20% concentration. The LC50 and LC95 values were determined as 6.875% and 17.306%, respectively. The extract caused a significant reduction (P < 0.05) in egg mass production at 10% concentration. In larval packet test, the extract was lethal to larvae in the concentrations of 10% and 20% after 24 h, with LC50 and LC95 values of 1.321% and 4.935%, respectively. Scopolamine and atropine showed 93.3% and 60.0% mortality of adult ticks, respectively, but they caused complete (100%) blocking of hatching as well as 100% larval mortality. Scopolamine and atropine were observed to be more potent than the crude extract at an equivalent concentration in both the bioassays.
评估了颠茄甲醇提取物及其成分东莨菪碱和阿托品对微小牛蜱的体外药效。提取物使用了5种浓度（1.25%、2.5%、5%、10%和20%），而东莨菪碱和阿托品均以0.1%的浓度进行测试。在成虫浸泡试验中，提取物在20%浓度下对蜱具有致死性。LC50和LC95值分别确定为6.875%和17.306%。提取物在10%浓度下可显著减少（P < 0.05）产卵量。在幼虫包囊试验中，提取物在10%和20%浓度下24小时后对幼虫具有致死性，LC50和LC95值分别为1.321%和4.935%。东莨菪碱和阿托品分别使成虫蜱的死亡率达到93.3%和60.0%，但它们导致孵化完全受阻（100%）以及幼虫死亡率达100%。在两种生物测定中，观察到在等效浓度下，东莨菪碱和阿托品比粗提取物更有效。
