Goes Annya Costa Araujo de Macedo, Pinto Francisco Maxwell Martins, Fernandes Guilherme Cardoso, Barbosa Jessica Sales, Correia Emanuela Santos, Ribeiro Ronaldo Albuquerque, Guimaraes Sergio Botelho, Lima Júnior Roberto Cesar Pereira, Brito Gerly Anne de Castro, Rodrigues Lusmar Veras
Department of Surgery, Federal University of Ceara, Fortaleza, CE, Brazil.
School of Pharmacy, UFC, Fortaleza, CE, Brazil.
Acta Cir Bras. 2014 Dec;29(12):787-93. doi: 10.1590/S0102-86502014001900004.
To study the anti-inflammatory actions of electroacupuncture (EAc) on an experimental colitis model in mice.
Thirty-eight male Swiss mice, divided in five groups, were subjected to induction of colitis by TNBS in 50% ethanol. Saline (SAL) and ethanol (ETNL) groups served as controls. TNBS+EAc and TNBS+ dexamethasone subgroups were treated with EAc 100Hz and dexamethasone (DEXA) 1 mg/Kg/day, respectively. After three days, a colon segment was obtained for quantification of myeloperoxidase (MPO) activity, immunohistochemistry for iNOS, malondialdehyde (MDA) and cytokines (IL-1β and IL-10).
Neutrophilic activity, assayed as MPO activity, was significantly higher in the TNBS colitis group than that in the saline control group. TNBS+EAc group showed suppression of IL-10 in the colon. EAc treatment significantly reduced the concentration of MDA and the expression of iNOS, as compared to the other groups.
Electroacupuncture 100Hz applied to acupoint ST-36 promotes an anti-inflammatory action on the TNBS-induced colitis, mediated by increase of IL-10 and decrease of iNOS expression.
研究电针对小鼠实验性结肠炎模型的抗炎作用。
38只雄性瑞士小鼠分为五组，通过在50%乙醇中注射三硝基苯磺酸（TNBS）诱导结肠炎。生理盐水（SAL）组和乙醇（ETNL）组作为对照组。TNBS+电针组和TNBS+地塞米松亚组分别接受100Hz电针和1mg/kg/天的地塞米松（DEXA）治疗。三天后，获取结肠段用于定量髓过氧化物酶（MPO）活性、诱导型一氧化氮合酶（iNOS）免疫组织化学、丙二醛（MDA）和细胞因子（IL-1β和IL-10）检测。
以MPO活性测定的中性粒细胞活性在TNBS结肠炎组显著高于生理盐水对照组。TNBS+电针组结肠中IL-10受到抑制。与其他组相比，电针治疗显著降低了MDA浓度和iNOS表达。
针刺足三里穴施加100Hz电针可对TNBS诱导的结肠炎产生抗炎作用，其机制是通过增加IL-10和降低iNOS表达介导的。
