Sohal R S, Svensson I, Sohal B H, Brunk U T
Department of Pathology II, University of Linköping, Sweden.
Mech Ageing Dev. 1989 Aug;49(2):129-35. doi: 10.1016/0047-6374(89)90096-1.
The general objective of this study was to examine the relationship between oxygen free radicals and the aging process. The rate of superoxide anion radical (O2.-) generation was measured in liver sub-mitochondrial particles from mouse, rat, rabbit, pig and cow, and in flight muscle sub-mitochondrial particles from the housefly. The rate of O2.- generation was determined as superoxide dismutase inhibitable reduction of ferricytochrome c in the presence of antimycin A and KCN. O2.- generation was inversely related to maximum species life span potential (MLSP) (r = -0.92). A 24-fold difference in the rate of O2.- production was observed between the cow and the fly while a 6-fold difference existed among the mammals. The results are interpreted to indicate that under identical conditions, mitochondria from organisms with low MLSP have a relatively greater propensity to generate O2.-. This may be suggestive of innate differences in the molecular organization of the inner mitochondrial membrane among different species.
本研究的总体目标是检验氧自由基与衰老过程之间的关系。测定了小鼠、大鼠、兔子、猪和牛肝脏亚线粒体颗粒以及家蝇飞行肌亚线粒体颗粒中超氧阴离子自由基（O2.-）的生成速率。在抗霉素A和氰化钾存在的情况下，通过超氧化物歧化酶可抑制的铁细胞色素c还原反应来确定O2.-的生成速率。O2.-的生成与最大物种寿命潜能（MLSP）呈负相关（r = -0.92）。牛和家蝇之间O2.-产生速率存在24倍的差异，而哺乳动物之间存在6倍的差异。这些结果被解释为表明在相同条件下，MLSP较低的生物体的线粒体产生O2.-的倾向相对更大。这可能暗示了不同物种线粒体内膜分子组织的固有差异。
