Bu Ying-Yue, Yamazaki Hiroyuki, Ukai Kazuyo, Namikoshi Michio
Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan.
Mar Drugs. 2014 Dec 17;12(12):6102-12. doi: 10.3390/md12126102.
Five new nucleoside antibiotics, named streptcytosines A-E (1-5), and six known compounds, de-amosaminyl-cytosamine (6), plicacetin (7), bamicetin (8), amicetin (9), collismycin B (10), and SF2738 C (11), were isolated from a culture broth of Streptomyces sp. TPU1236A collected in Okinawa, Japan. The structures of new compounds were elucidated on the basis of their spectroscopic data (HRFABMS, IR, UV, and 2D NMR experiments including 1H-1H COSY, HMQC, HMBC, and NOESY spectra). Streptcytosine A (1) belonged to the amicetin group antibiotics, and streptcytosines B-E (2-5) were derivatives of de-amosaminyl-cytosamine (6), 2,3,6-trideoxyglucopyranosyl cytosine. Compound 1 inhibited the growth of Mycobacterium smegmatis (MIC = 32 µg/mL), while compounds 2-5 were not active at 50 µg/disc. Bamicetin (8) and amicetin (9) showed the MICs of 16 and 8 µg/mL, respectively.
从日本冲绳采集的链霉菌属TPU1236A的培养液中分离出5种新的核苷类抗生素，命名为链霉菌胞嘧啶A - E（1 - 5），以及6种已知化合物，去氨基氨甲酰胞嘧啶（6）、plicacetin（7）、巴米霉素（8）、阿米西丁（9）、柱晶白霉素B（10）和SF2738 C（11）。根据新化合物的光谱数据（高分辨快原子轰击质谱、红外光谱、紫外光谱以及二维核磁共振实验，包括1H - 1H COSY、HMQC、HMBC和NOESY谱）阐明了它们的结构。链霉菌胞嘧啶A（1）属于阿米西丁类抗生素，链霉菌胞嘧啶B - E（2 - 5）是去氨基氨甲酰胞嘧啶（6）、2,3,6 - 三脱氧葡萄糖吡喃糖基胞嘧啶的衍生物。化合物1抑制耻垢分枝杆菌的生长（最小抑菌浓度 = 32 μg/mL），而化合物2 - 5在50 μg/圆片时无活性。巴米霉素（8）和阿米西丁（9）的最小抑菌浓度分别为16和8 μg/mL。
