Anwar Nusratgul, Rozahon Manziram, Zayadan Bolatkhan, Mamtimin Hormathan, Abdurahman Mehfuzem, Kurban Marygul, Abdurusul Mihribangul, Mamtimin Tursunay, Abdukerim Muhtar, Rahman Erkin
College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4372-4378. doi: 10.1099/ijsem.0.002295. Epub 2017 Oct 6.
An endophytic bacterium, MA-69, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69 was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69 was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5 % to Pseudomonas azotifigens JCM 12708, followed by Pseudomonas matsuisoli JCM 30078 (97.5 %), Pseudomonas balearica DSM 6083 (97.1 %), Azotobacter salinestris ATCC 49674 (96.1 %) and Pseudomonas indica DSM 14015 (95.9 %). Analysis of strain MA-69 based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas. The DNA G+C content of strain MA-69 was 64.1 mol%. DNA-DNA hybridization with Pseudomonas azotifigens JCM 12708, Pseudomonas matsuisoli JCM 30078 and Pseudomonas balearica DSM 6083 revealed 62.9, 60.1 and 49.0 % relatedness, respectively. The major fatty acids in strain MA-69 were summed feature 3 (25.7 %), summed feature 8 (24.0 %), C19 : 0cyclo ω8c (19.9 %), C16 : 0 (14.6 %) and C12 : 0 (6.3 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69 (=CCTCC AB 2013065=KCTC 42447).
从中国新疆乌干河古河道胡杨（Populuseuphratica）树茎干的贮藏液中分离出一株内生细菌MA-69。菌株MA-69呈短杆状，革兰氏染色阴性，不产芽孢，好氧，具单极鞭毛，能运动。基于16S rRNA基因序列的系统发育分析表明，菌株MA-69归属于假单胞菌属（Pseudomonas），其16S rRNA基因序列与固氮假单胞菌（Pseudomonas azotifigens）JCM 12708的相似性最高，为97.5%，其次是松岛假单胞菌（Pseudomonas matsuisoli）JCM 30078（97.5%）、巴利阿里假单胞菌（Pseudomonas balearica）DSM 6083（97.1%）、盐生固氮菌（Azotobacter salinestris）ATCC 49674（96.1%）和印度假单胞菌（Pseudomonas indica）DSM 14015（95.9%）。基于rpoB、rpoD和gyrB这三个持家基因对菌株MA-69进行分析，进一步证实该分离株与假单胞菌属的其他种明显不同。菌株MA-69的DNA G+C含量为64.1 mol%。与固氮假单胞菌JCM 12708、松岛假单胞菌JCM 30078和巴利阿里假单胞菌DSM 6083的DNA-DNA杂交结果显示，其亲缘关系分别为62.9%、60.1%和49.0%。菌株MA-69的主要脂肪酸为特征性总和3（25.7%）、特征性总和8（24.0%）、C19 : 0环ω8c（19.9%）、C16 : 0（14.6%）和C12 : 0（6.3%）。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。泛醌Q-9是菌株MA-69的主要醌类。基于表型、化学分类和系统发育特性，菌株MA-69代表假单胞菌属的一个新种，提议将其命名为塔里木假单胞菌（Pseudomonas tarimensis）sp. nov.。模式菌株为MA-69（=CCTCC AB 2013065=KCTC 42447）。
