Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan.
Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2330-2337. doi: 10.1099/ijs.0.060319-0. Epub 2014 Apr 17.
Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11(T) and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7% mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2% sequence similarity to strains of other species of the genus Pseudomonas (95.7-95.9% similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5% of the total) were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), C16 : 1ω6c and/or C16 : 1ω7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11(T) and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA-DNA hybridization obtained between the two strains was 79.0% (reciprocal value 89.4% using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA-DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11(T) and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas. Thus, the strains are proposed to represent a novel species of Pseudomonas, for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11(T) ( = JCM 19513(T) = BCRC 80696(T)); a second strain of the same species is CC-AMHZ-5 ( = JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis are also proposed.
两株革兰氏阴性、需氧、杆状、不产芽孢的细菌菌株，通过单极鞭毛运动，分别命名为 CC-AMH-11(T) 和 CC-AMHZ-5，从台湾花莲海岸的海滨鸟类粪便中分离得到。这些菌株的 16S rRNA 基因序列相似度为 99.7%，而与假单胞菌属的其他种的菌株相似度<96.2%（与模式种铜绿假单胞菌 LMG 1242T 的相似度为 95.7-95.9%），并且在系统发育树中形成了一个独特的共同进化枝。共同的主要脂肪酸（占总脂肪酸的>5%）为 C18:1ω7c 和/或 C18:1ω6c（特征 8 总和）、C16:1ω6c 和/或 C16:1ω7c（特征 3 总和）、C16:0 和 C12:0。检测到磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰丝氨酸、一种未鉴定的脂质和一种未鉴定的磷脂作为共同的极性脂。菌株 CC-AMH-11(T) 和 CC-AMHZ-5 的 DNA G+C 含量分别为 61.1 和 61.6 mol%。共同的主要呼吸醌是泛醌 9（Q-9），主要的多胺是腐胺。两株菌之间获得的 DNA-DNA 杂交率为 79.0%（用 CC-AMHZ-5 DNA 作为探针时的倒数值为 89.4%）。CC-AMH-11(T) 和 CC-AMHZ-5 之间非常高的 16S rRNA 基因序列相似度和 DNA-DNA 相关性以及难以区分的表型特征清楚地表明，它们是单一基因组种的两个不同菌株。然而，这些菌株还表现出一些基因型和表型特征，使它们与其他密切相关的假单胞菌种区别开来。因此，这些菌株被提议代表假单胞菌属的一个新种，建议将其命名为 Pseudomonas hussainii sp. nov. 模式株为 CC-AMH-11(T)（=JCM 19513(T)=BCRC 80696(T)）；同一物种的第二株菌株为 CC-AMHZ-5（=JCM 19512=BCRC 80697）。此外，还提出了对 Pohangensis 种、苯并类生菌属和芥菜假单胞菌属的描述进行修正。
