Khan A U
Department of Chemistry, Harvard University, Cambridge, MA 02138.
J Biolumin Chemilumin. 1989 Jul;4(1):200-7. doi: 10.1002/bio.1170040129.
Singlet oxygen generation is reported from (1) enzymatic reaction and (2) electron transfer reactions of the superoxide anion measured directly with an ultrasensitive near-IR emission spectrophotometer by monitoring the O2(1 delta g)----O2 (3 sigma g-) transition at 1268 nm. Near-IR emission spectra from the myeloperoxidase and lactoperoxidase enzymatic systems show only emission of singlet oxygen at 1268 nm. The lipoxygenase/Na-linoleate enzymatic reaction exhibits two emissions, 1268 nm and 1288 nm. The latter emission is identified as originating from a peroxy radical. Spectral and kinetic data giving evidence of singlet oxygen generation is obtained from the reaction of potassium superoxide solubilized by 18-crown-6-ether in acetonitrile with a series of organometallic coordination compounds.
据报道，单线态氧的产生来自：（1）酶促反应；（2）超氧阴离子的电子转移反应，通过用超灵敏近红外发射分光光度计监测1268nm处O2(1Δg)→O2(3Σg-)跃迁，直接对其进行测量。髓过氧化物酶和乳过氧化物酶酶促系统的近红外发射光谱仅显示在1268nm处有单线态氧的发射。脂氧合酶/亚油酸钠酶促反应表现出两种发射，分别在1268nm和1288nm处。后者的发射被确定源自过氧自由基。通过18-冠-6-醚在乙腈中溶解的超氧化钾与一系列有机金属配位化合物的反应，获得了证明单线态氧产生的光谱和动力学数据。
