Tang Qingfa, Shao Meng, Wang Ying, Zhao Huinan, Fan Chunlin, Huang Xiaojun, Li Yaolan, Ye Wencai
School of Chinese Medical Sciences, Southern Medical University, Guangzhou, PR China.
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
J Chromatogr Sci. 2015 Jul;53(6):963-7. doi: 10.1093/chromsci/bmu160. Epub 2014 Dec 19.
A high-performance liquid chromatography method coupled with diode array detection (HPLC-DAD) was developed for simultaneous determination of two coumarins and eight flavonoids in Lophatherum gracile Brongn (Gramineae), namely 5-O-coumaroylquinic acid (i), 4-O-coumaroylquinic acid (ii), luteolin 6-C-β-d-galactopyranosiduronic acid (1→2)-β-d-glucopyranoside (iii), 7-O-β-d-glucopyranosyl-6-C-α-l-arabinopy ranoside (iv), isoorientin (v), swertiajaponin (vi), luteolin 6-C-β-d-galactopyranosiduronic acid (1→2)-α-l-arabinopyranoside (vii), Saponaretin (viii), swertisin (ix) and apigenin 6-C-β-d-galactopyranosiduronic acid (1→2)-α-l-arabinopyranoside (x). The analysis was performed on Cosmosil MS-IIⅡ C18 column (250 × 4.6 mm, 5 µm) with gradient elution of 0.1% aqueous acetic acid and acetonitrile. The detection wavelength was 330 nm. The developed method was able to determine the bioactive compounds with excellent resolution, precision and recovery. The validated method was successfully applied for the analysis of the 10 bioactive compounds in n samples from different cultivated regions. The results indicated that the developed method can be used as a suitable quality control method for L. gracile.
建立了一种高效液相色谱-二极管阵列检测法（HPLC-DAD），用于同时测定淡竹叶（禾本科）中的两种香豆素和八种黄酮类化合物，即5-O-香豆酰奎宁酸（i）、4-O-香豆酰奎宁酸（ii）、木犀草素6-C-β-D-吡喃半乳糖醛酸（1→2）-β-D-吡喃葡萄糖苷（iii）、7-O-β-D-吡喃葡萄糖基-6-C-α-L-阿拉伯吡喃糖苷（iv）、异荭草苷（v）、獐牙菜苷（vi）、木犀草素6-C-β-D-吡喃半乳糖醛酸（1→2）-α-L-阿拉伯吡喃糖苷（vii）、肥皂草素（viii）、獐牙菜黄素（ix）和芹菜素6-C-β-D-吡喃半乳糖醛酸（1→2）-α-L-阿拉伯吡喃糖苷（x）。分析在Cosmosil MS-IIⅡ C18柱（250×4.6 mm，5 µm）上进行，采用0.1%乙酸水溶液和乙腈梯度洗脱。检测波长为330 nm。所建立的方法能够以优异的分离度、精密度和回收率测定生物活性化合物。该验证方法成功应用于来自不同种植区域的n个样品中10种生物活性化合物的分析。结果表明，所建立的方法可作为淡竹叶合适的质量控制方法。
